EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of Vps4 with circular peptides and implications for translocation of two polypeptide chains by AAA+ ATPases.
ジャーナル・号・ページ	Elife, Vol. 8, Year 2019
掲載日	2019年6月11日
著者	Han Han / James M Fulcher / Venkata P Dandey / Janet H Iwasa / Wesley I Sundquist / Michael S Kay / Peter S Shen / Christopher P Hill /
PubMed 要旨	Many AAA+ ATPases form hexamers that unfold protein substrates by translocating them through their central pore. Multiple structures have shown how a helical assembly of subunits binds a single ...Many AAA+ ATPases form hexamers that unfold protein substrates by translocating them through their central pore. Multiple structures have shown how a helical assembly of subunits binds a single strand of substrate, and indicate that translocation results from the ATP-driven movement of subunits from one end of the helical assembly to the other end. To understand how more complex substrates are bound and translocated, we demonstrated that linear and cyclic versions of peptides bind to the AAA+ ATPase Vps4 with similar affinities, and determined cryo-EM structures of cyclic peptide complexes. The peptides bind in a hairpin conformation, with one primary strand equivalent to the single chain peptide ligands, while the second strand returns through the translocation pore without making intimate contacts with Vps4. These observations indicate a general mechanism by which AAA+ ATPases may translocate a variety of substrates that include extended chains, hairpins, and crosslinked polypeptide chains.
リンク	Elife / PubMed:31184588 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 4.4 Å
構造データ	0443 6ndy EMDB-0443, PDB-6ndy: Vps4 with Cyclic Peptide Bound in the Central Pore 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-20139: Vps4 with Cyclic Peptide Bound in the Central Pore (Focused Classification of Subunit F, State1) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-20140: Vps4 with Cyclic Peptide Bound in the Central Pore (Focused Classification of Subunit F, State2) 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-20141: Vps4 with Cyclic Peptide Bound in the Central Pore (Focused Classification of Subunit F, State3) 手法: EM (単粒子) / 解像度: 4.3 Å 20142 6oo2 EMDB-20142, PDB-6oo2: Vps4 with Cyclic Peptide Bound in the Central Pore 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-20144: Vps4 with Cyclic Peptide Bound in the Central Pore (Map for Peptide cF30) 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-20147: Vps4 with Cyclic Peptide Bound in the Central Pore (Map for Peptide cFF30) 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母) synthetic construct (人工物)
キーワード	TRANSPORT PROTEIN/PEPTIDE / Vps4 / ESCRT (ESCRT) / Vta1 / AAA ATPase / TRANSPORT PROTEIN (運搬体タンパク質) / TRANSPORT PROTEIN-PEPTIDE complex