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-Structure paper
タイトル | cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud. |
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ジャーナル・号・ページ | J Struct Biol, Vol. 203, Issue 3, Page 230-235, Year 2018 |
掲載日 | 2018年6月1日 |
著者 | Michael A Cianfrocco / Indrajit Lahiri / Frank DiMaio / Andres E Leschziner / |
PubMed 要旨 | Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM ...Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2 Å structure of ß-galactosidase in ∼55 h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud. |
リンク | J Struct Biol / PubMed:29864529 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.2 Å |
構造データ | |
由来 |
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キーワード | HYDROLASE (加水分解酵素) / cryo-EM (低温電子顕微鏡法) / Amazon Web Services / RELION |