EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus.
ジャーナル・号・ページ	Nat Commun, Vol. 8, Page 15408, Year 2017
掲載日	2017年5月23日
著者	Abhay Kotecha / Quan Wang / Xianchi Dong / Serban L Ilca / Marina Ondiviela / Rao Zihe / Julian Seago / Bryan Charleston / Elizabeth E Fry / Nicola G A Abrescia / Timothy A Springer / Juha T Huiskonen / David I Stuart /
PubMed 要旨	Foot-and-mouth disease virus (FMDV) mediates cell entry by attachment to an integrin receptor, generally αvβ6, via a conserved arginine-glycine-aspartic acid (RGD) motif in the exposed, antigenic, ...Foot-and-mouth disease virus (FMDV) mediates cell entry by attachment to an integrin receptor, generally αvβ6, via a conserved arginine-glycine-aspartic acid (RGD) motif in the exposed, antigenic, GH loop of capsid protein VP1. Infection can also occur in tissue culture adapted virus in the absence of integrin via acquired basic mutations interacting with heparin sulphate (HS); this virus is attenuated in natural infections. HS interaction has been visualized at a conserved site in two serotypes suggesting a propensity for sulfated-sugar binding. Here we determined the interaction between αvβ6 and two tissue culture adapted FMDV strains by cryo-electron microscopy. In the preferred mode of engagement, the fully open form of the integrin, hitherto unseen at high resolution, attaches to an extended GH loop via interactions with the RGD motif plus downstream hydrophobic residues. In addition, an N-linked sugar of the integrin attaches to the previously identified HS binding site, suggesting a functional role.
リンク	Nat Commun / PubMed:28534487 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.3 - 12.3 Å
構造データ	3630 5ned EMDB-3630, PDB-5ned: CryoEM Structure of Foot and Mouth Disease Virus O PanAsia 手法: EM (単粒子) / 解像度: 3.1 Å 3631 5nej EMDB-3631, PDB-5nej: CryoEM Structure of Foot and Mouth Disease Virus O1 Manisa 手法: EM (単粒子) / 解像度: 3.1 Å 3632 5nem EMDB-3632, PDB-5nem: Localised reconstruction of alpha v beta 6 bound to Foot and Mouth Disease Virus O PanAsia - Pose A. 手法: EM (単粒子) / 解像度: 10.8 Å 3633 5ner EMDB-3633, PDB-5ner: Localised reconstruction of alpha v beta 6 bound to Foot and Mouth Disease Virus O PanAsia - Pose A prime. 手法: EM (単粒子) / 解像度: 11.5 Å 3634 5net EMDB-3634, PDB-5net: Localised Reconstruction of Integrin alpha V beta 6 bound to Foot and Mouth Disease Virus O1 Manisa - Pose A. 手法: EM (単粒子) / 解像度: 8.6 Å 3635 5neu EMDB-3635, PDB-5neu: Localised Reconstruction of Integrin alpha V beta 6 bound to Foot and Mouth Disease Virus O1 Manisa - Pose B. 手法: EM (単粒子) / 解像度: 12.3 Å PDB-5ne4: Crystal Structure of Foot and Mouth Disease Virus O PanAsia 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å
化合物	ChemComp-HOH: WATER / 水 ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス / マンノース ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	foot-and-mouth disease virus (口蹄疫ウイルス) homo sapiens (ヒト) foot-and-mouth disease virus - type o (口蹄疫ウイルス)
キーワード	VIRUS (ウイルス) / Foot and Mouth Disease Virus (口蹄疫ウイルス) / FMDV (口蹄疫ウイルス) / OpanAsia / virus-receptor / complex