EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes.
ジャーナル・号・ページ	Cell, Vol. 167, Issue 5, Page 1229-1240.e15, Year 2016
掲載日	2016年11月17日
著者	Sichen Shao / Jason Murray / Alan Brown / Jack Taunton / V Ramakrishnan / Ramanujan S Hegde /
PubMed 要旨	In eukaryotes, accurate protein synthesis relies on a family of translational GTPases that pair with specific decoding factors to decipher the mRNA code on ribosomes. We present structures of the ...In eukaryotes, accurate protein synthesis relies on a family of translational GTPases that pair with specific decoding factors to decipher the mRNA code on ribosomes. We present structures of the mammalian ribosome engaged with decoding factor⋅GTPase complexes representing intermediates of translation elongation (aminoacyl-tRNA⋅eEF1A), termination (eRF1⋅eRF3), and ribosome rescue (Pelota⋅Hbs1l). Comparative analyses reveal that each decoding factor exploits the plasticity of the ribosomal decoding center to differentially remodel ribosomal proteins and rRNA. This leads to varying degrees of large-scale ribosome movements and implies distinct mechanisms for communicating information from the decoding center to each GTPase. Additional structural snapshots of the translation termination pathway reveal the conformational changes that choreograph the accommodation of decoding factors into the peptidyl transferase center. Our results provide a structural framework for how different states of the mammalian ribosome are selectively recognized by the appropriate decoding factor⋅GTPase complex to ensure translational fidelity.
リンク	Cell / PubMed:27863242 / PubMed Central
手法	EM (単粒子)
解像度	3.06 - 3.99 Å
構造データ	EMDB-4129: Structure of the mammalian ribosome with P- and E-site tRNAs and an unoccupied A site 手法: EM (単粒子) / 解像度: 3.06 Å 4130 5lzs EMDB-4130, PDB-5lzs: Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B 手法: EM (単粒子) / 解像度: 3.31 Å 4131 5lzt EMDB-4131, PDB-5lzt: Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. 手法: EM (単粒子) / 解像度: 3.65 Å 4132 5lzu EMDB-4132, PDB-5lzu: Structure of the mammalian ribosomal termination complex with accommodated eRF1 手法: EM (単粒子) / 解像度: 3.75 Å 4133 5lzv EMDB-4133, PDB-5lzv: Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. 手法: EM (単粒子) / 解像度: 3.35 Å 4134 5lzw EMDB-4134, PDB-5lzw: Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. 手法: EM (単粒子) / 解像度: 3.53 Å 4135 5lzx EMDB-4135, PDB-5lzx: Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. 手法: EM (単粒子) / 解像度: 3.67 Å 4136 5lzy EMDB-4136, PDB-5lzy: Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. 手法: EM (単粒子) / 解像度: 3.99 Å 4137 5lzz EMDB-4137, PDB-5lzz: Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) 手法: EM (単粒子) / 解像度: 3.47 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-7C4: (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide ChemComp-GCP: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / グアニリルメチレンジホスホン酸 / GMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター
由来	oryctolagus cuniculus (ウサギ) Rabbit (ウサギ) homo sapiens (ヒト)
キーワード	RIBOSOME (リボソーム) / Translation (翻訳) / Elongation