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-Structure paper
タイトル | Assembly, maturation and three-dimensional helical structure of the teratogenic rubella virus. |
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ジャーナル・号・ページ | PLoS Pathog, Vol. 13, Issue 6, Page e1006377, Year 2017 |
掲載日 | 2017年6月2日 |
著者 | Vidya Mangala Prasad / Thomas Klose / Michael G Rossmann / |
PubMed 要旨 | Viral infections during pregnancy are a significant cause of infant morbidity and mortality. Of these, rubella virus infection is a well-substantiated example that leads to miscarriages or severe ...Viral infections during pregnancy are a significant cause of infant morbidity and mortality. Of these, rubella virus infection is a well-substantiated example that leads to miscarriages or severe fetal defects. However, structural information about the rubella virus has been lacking due to the pleomorphic nature of the virions. Here we report a helical structure of rubella virions using cryo-electron tomography. Sub-tomogram averaging of the surface spikes established the relative positions of the viral glycoproteins, which differed from the earlier icosahedral models of the virus. Tomographic analyses of in vitro assembled nucleocapsids and virions provide a template for viral assembly. Comparisons of immature and mature virions show large rearrangements in the glycoproteins that may be essential for forming the infectious virions. These results present the first known example of a helical membrane-enveloped virus, while also providing a structural basis for its assembly and maturation pathway. |
リンク | PLoS Pathog / PubMed:28575072 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 11.1 - 35.0 Å |
構造データ | EMDB-8248: Subtomogram-averaged surface glycoprotein spike density of rubella virus EMDB-8249: Subtomogram-averaged map of a single rubella virus capsid unit EMDB-8250: Subtomogram-averaged map of a single rubella virus capsid unit EMDB-8251: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | VIRAL PROTEIN (ウイルスタンパク質) / Rubella virus (風疹ウイルス) / surface glycoprotein spike / E1-E2 heterodimer / rubella virus capsid protein |