EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of BipA in GTP form bound to the ratcheted ribosome.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 112, Issue 35, Page 10944-10949, Year 2015
掲載日	2015年9月1日
著者	Veerendra Kumar / Yun Chen / Rya Ero / Tofayel Ahmed / Jackie Tan / Zhe Li / Andrew See Weng Wong / Shashi Bhushan / Yong-Gui Gao /
PubMed 要旨	BPI-inducible protein A (BipA) is a member of the family of ribosome-dependent translational GTPase (trGTPase) factors along with elongation factors G and 4 (EF-G and EF4). Despite being highly ...BPI-inducible protein A (BipA) is a member of the family of ribosome-dependent translational GTPase (trGTPase) factors along with elongation factors G and 4 (EF-G and EF4). Despite being highly conserved in bacteria and playing a critical role in coordinating cellular responses to environmental changes, its structures (isolated and ribosome bound) remain elusive. Here, we present the crystal structures of apo form and GTP analog, GDP, and guanosine-3',5'-bisdiphosphate (ppGpp)-bound BipA. In addition to having a distinctive domain arrangement, the C-terminal domain of BipA has a unique fold. Furthermore, we report the cryo-electron microscopy structure of BipA bound to the ribosome in its active GTP form and elucidate the unique structural attributes of BipA interactions with the ribosome and A-site tRNA in the light of its possible function in regulating translation.
リンク	Proc Natl Acad Sci U S A / PubMed:26283392 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.31 - 5 Å
構造データ	6396 5a9z EMDB-6396: The structure of BipA in GTP form bound to the ratcheted ribosome PDB-5a9z: Complex of Thermous thermophilus ribosome bound to BipA-GDPCP 手法: EM (単粒子) / 解像度: 4.7 Å 6397 5aa0 EMDB-6397: The structure of BipA in GTP form bound to the ratcheted ribosome PDB-5aa0: Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP 手法: EM (単粒子) / 解像度: 4.8 Å PDB-5a9v: Structure of apo BipA 手法: X-RAY DIFFRACTION / 解像度: 3.31 Å PDB-5a9w: Structure of GDPCP BipA 手法: X-RAY DIFFRACTION / 解像度: 3.7 Å PDB-5a9x: Structure of GDP bound BipA 手法: X-RAY DIFFRACTION / 解像度: 3.8 Å PDB-5a9y: Structure of ppGpp BipA 手法: X-RAY DIFFRACTION / 解像度: 4.0 Å
化合物	ChemComp-GCP: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / グアニリルメチレンジホスホン酸 / GMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-HOH: WATER / 水 ChemComp-G4P: GUANOSINE-5',3'-TETRAPHOSPHATE / ppGpp / Guanosine pentaphosphate ChemComp-NMY: NEOMYCIN / ネオマイシンB / 抗生剤YM / ネオマイシン ChemComp-8AN*: 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) / 3′-アミノ-3′-デオキシアデノシン5′-りん酸
由来	Thermus thermophilus (サーマス・サーモフィルス) escherichia coli (大腸菌) thermus thermophilus hb8 (サーマス・サーモフィルス)
キーワード	RIBOSOMAL PROTEIN / RIBOSOME (リボソーム) / TRANSLATIONAL GTPASE FACTORS / BIPA / X-RAY CRYSTALLOGRAPHY AND CRYO-ELECTRON MICROSCOPY / PROTEIN (タンパク質)