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-Structure paper
タイトル | Atomic model of an infectious rotavirus particle. |
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ジャーナル・号・ページ | EMBO J, Vol. 30, Issue 2, Page 408-416, Year 2011 |
掲載日 | 2011年1月19日 |
著者 | Ethan C Settembre / James Z Chen / Philip R Dormitzer / Nikolaus Grigorieff / Stephen C Harrison / |
PubMed 要旨 | Non-enveloped viruses of different types have evolved distinct mechanisms for penetrating a cellular membrane during infection. Rotavirus penetration appears to occur by a process resembling ...Non-enveloped viruses of different types have evolved distinct mechanisms for penetrating a cellular membrane during infection. Rotavirus penetration appears to occur by a process resembling enveloped-virus fusion: membrane distortion linked to conformational changes in a viral protein. Evidence for such a mechanism comes from crystallographic analyses of fragments of VP4, the rotavirus-penetration protein, and infectivity analyses of structure-based VP4 mutants. We describe here the structure of an infectious rotavirus particle determined by electron cryomicroscopy (cryoEM) and single-particle analysis at about 4.3 Å resolution. The cryoEM image reconstruction permits a nearly complete trace of the VP4 polypeptide chain, including the positions of most side chains. It shows how the two subfragments of VP4 (VP8(*) and VP5(*)) retain their association after proteolytic cleavage, reveals multiple structural roles for the β-barrel domain of VP5(*), and specifies interactions of VP4 with other capsid proteins. The virion model allows us to integrate structural and functional information into a coherent mechanism for rotavirus entry. |
リンク | EMBO J / PubMed:21157433 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.8 Å |
構造データ | |
化合物 | ChemComp-ZN: ChemComp-NAG: |
由来 |
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キーワード | VIRUS (ウイルス) / Rotavirus (ロタウイルス) / Triple Layered Particle / Near Atomic Resolution / VP2 / VP6 / VP4 / VP7 / Double layered particle / de novo (De novo) / Infectious (感染) / DLP / ICOSAHEDRAL VIRUS |