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-Structure paper
タイトル | Structure of the mammalian 80S ribosome at 8.7 A resolution. |
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ジャーナル・号・ページ | Structure, Vol. 16, Issue 4, Page 535-548, Year 2008 |
掲載日 | 2008年6月16日 |
著者 | Preethi Chandramouli / Maya Topf / Jean-François Ménétret / Narayanan Eswar / Jamie J Cannone / Robin R Gutell / Andrej Sali / Christopher W Akey / |
PubMed 要旨 | In this paper, we present a structure of the mammalian ribosome determined at approximately 8.7 A resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was ...In this paper, we present a structure of the mammalian ribosome determined at approximately 8.7 A resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was created by docking homology models of subunit rRNAs and conserved proteins into the density map. We then modeled expansion segments in the subunit rRNAs and found unclaimed density for approximately 20 proteins. In general, many conserved proteins and novel proteins interact with expansion segments to form an integrated framework that may stabilize the mature ribosome. Our structure provides a snapshot of the mammalian ribosome at the beginning of translation and lends support to current models in which large movements of the small subunit and L1 stalk occur during tRNA translocation. Finally, details are presented for intersubunit bridges that are specific to the eukaryotic ribosome. We suggest that these bridges may help reset the conformation of the ribosome to prepare for the next cycle of chain elongation. |
リンク | Structure / PubMed:18400176 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 8.7 Å |
構造データ | |
由来 |
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キーワード | RIBOSOMAL PROTEIN/RNA (リボソーム) / protein-RNA complex / 40S ribosomal subunit (リボソーム) / RIBOSOMAL PROTEIN-RNA COMPLEX (リボソーム) |