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-Structure paper
タイトル | Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements. |
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ジャーナル・号・ページ | Mol Cell, Vol. 22, Issue 4, Page 533-543, Year 2006 |
掲載日 | 2006年5月19日 |
著者 | Kakoli Mitra / Christiane Schaffitzel / Felcy Fabiola / Michael S Chapman / Nenad Ban / Joachim Frank / |
PubMed 要旨 | In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models ...In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA.(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors. |
リンク | Mol Cell / PubMed:16713583 |
手法 | EM (単粒子) |
解像度 | 15 Å |
構造データ | PDB-4v4v: PDB-4v4w: |
由来 |
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キーワード | RIBOSOME (リボソーム) / SecM; nascent chain; signal transduction; RNA world; polypeptide exit tunnel; translocation; ribosome; elongation arrest / SecM; nascent chain; signal transduction; RNA world; polypeptide exit tunnel; translocation; ribosome; elongation arrest; protein-conducting channel |