EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.
ジャーナル・号・ページ	Cell, Vol. 158, Issue 5, Page 1123-1135, Year 2014
掲載日	2014年8月28日
著者	Jan P Erzberger / Florian Stengel / Riccardo Pellarin / Suyang Zhang / Tanja Schaefer / Christopher H S Aylett / Peter Cimermančič / Daniel Boehringer / Andrej Sali / Ruedi Aebersold / Nenad Ban /
PubMed 要旨	Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, ...Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40S⋅eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.
リンク	Cell / PubMed:25171412 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2 - 28.1 Å
構造データ	EMDB-2670: Occupied class from the supervised classification of a negatively stained Lachancea kluyveri 40S-eIF1-eIF3 complex. 手法: EM (単粒子) / 解像度: 28.1 Å EMDB-2671: Unoccupied class from the supervised classification of a negatively stained Lachancea kluyveri 40S-eIF1-eIF3 complex. 手法: EM (単粒子) / 解像度: 24.6 Å PDB-3j8b: Model of the human eIF3 PCI-MPN octamer docked into the 43S-HCV IRES EM map 手法: ELECTRON MICROSCOPY / 解像度: 9.3 Å PDB-3j8c: Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map 手法: ELECTRON MICROSCOPY / 解像度: 11.6 Å PDB-4u1c: Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer 手法: X-RAY DIFFRACTION / 解像度: 3.5 Å PDB-4u1d: Structure of the PCI domain of translation initiation factor eIF3a 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å PDB-4u1e: Crystal structure of the eIF3b-CTD/eIF3i/eIF3g-NTD translation initiation complex 手法: X-RAY DIFFRACTION / 解像度: 2.0 Å PDB-4u1f: Crystal structure of middle domain of eukaryotic translation initiation factor eIF3b 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å
化合物	ChemComp-HOH: WATER / 水
由来	Lachancea kluyveri (菌類) homo sapiens (ヒト) saccharomyces cerevisiae (パン酵母)
キーワード	TRANSLATION (翻訳 (生物学)) / translation initiation / eIF3 complex (EIF3) / PCI-domains / PCI domain / beta-propeller (Βプロペラドメイン)