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-Structure paper
タイトル | 4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus. |
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ジャーナル・号・ページ | EMBO J, Vol. 30, Issue 18, Page 3854-3863, Year 2011 |
掲載日 | 2011年8月9日 |
著者 | Rui Zhang / Corey F Hryc / Yao Cong / Xiangan Liu / Joanita Jakana / Rodion Gorchakov / Matthew L Baker / Scott C Weaver / Wah Chiu / |
PubMed 要旨 | Venezuelan equine encephalitis virus (VEEV), a member of the membrane-containing Alphavirus genus, is a human and equine pathogen, and has been developed as a biological weapon. Using electron cryo- ...Venezuelan equine encephalitis virus (VEEV), a member of the membrane-containing Alphavirus genus, is a human and equine pathogen, and has been developed as a biological weapon. Using electron cryo-microscopy (cryo-EM), we determined the structure of an attenuated vaccine strain, TC-83, of VEEV to 4.4 Å resolution. Our density map clearly resolves regions (including E1, E2 transmembrane helices and cytoplasmic tails) that were missing in the crystal structures of domains of alphavirus subunits. These new features are implicated in the fusion, assembly and budding processes of alphaviruses. Furthermore, our map reveals the unexpected E3 protein, which is cleaved and generally thought to be absent in the mature VEEV. Our structural results suggest a mechanism for the initial stage of nucleocapsid core formation, and shed light on the virulence attenuation, host recognition and neutralizing activities of VEEV and other alphavirus pathogens. |
リンク | EMBO J / PubMed:21829169 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.8 Å |
構造データ | EMDB-5275: 4.4 Angstrom Cryo-EM Structure of an Enveloped Alphavirus Venezuelan Equine Encephalitis Virus EMDB-5276: 4.4 Angstrom Cryo-EM Structure of an Enveloped Alphavirus Venezuelan Equine Encephalitis Virus |
由来 |
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キーワード | VIRUS (ウイルス) / alphavirus / bioweapon / VEEV |