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-Structure paper
タイトル | The flavivirus precursor membrane-envelope protein complex: structure and maturation. |
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ジャーナル・号・ページ | Science, Vol. 319, Issue 5871, Page 1830-1834, Year 2008 |
掲載日 | 2008年3月28日 |
著者 | Long Li / Shee-Mei Lok / I-Mei Yu / Ying Zhang / Richard J Kuhn / Jue Chen / Michael G Rossmann / |
PubMed 要旨 | Many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of ...Many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of the precursor membrane protein (prM), turning inert virus into infectious particles. We have determined the 2.2 angstrom resolution crystal structure of a recombinant protein in which the dengue virus prM is linked to the envelope glycoprotein E. The structure represents the prM-E heterodimer and fits well into the cryo-electron microscopy density of immature virus at neutral pH. The pr peptide beta-barrel structure covers the fusion loop in E, preventing fusion with host cell membranes. The structure provides a basis for identifying the stages of its pH-directed conformational metamorphosis during maturation, ending with release of pr when budding from the host. |
リンク | Science / PubMed:18369147 |
手法 | X線回折 / EM (単粒子) |
解像度 | 2.2 - 12.5 Å |
構造データ | PDB-3c5x: PDB-3c6d: PDB-3c6e: |
化合物 | ChemComp-HOH: ChemComp-NDG: ChemComp-NAG: |
由来 |
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キーワード | VIRAL PROTEIN (ウイルスタンパク質) / BETA BARREL (Βバレル) / prM-E protein complex / Helicase (ヘリカーゼ) / Hydrolase (加水分解酵素) / Nucleotide-binding / RNA replication (RNA依存性RNAポリメラーゼ) / Transmembrane (膜貫通型タンパク質) / VIRUS (ウイルス) / icosahedral virion / ATP-binding / Capsid protein (カプシド) / Cleavage on pair of basic residues / Endoplasmic reticulum (小胞体) / Envelope protein (エンベロープ (ウイルス)) / Glycoprotein (糖タンパク質) / Metal-binding / Multifunctional enzyme / Nucleotidyltransferase / Nucleus (Nucleus) / Phosphoprotein / Protease (プロテアーゼ) / Ribonucleoprotein (核タンパク質) / RNA-binding / RNA-directed RNA polymerase (RNA依存性RNAポリメラーゼ) / Secreted (分泌) / Serine protease (セリンプロテアーゼ) / Transcription (転写 (生物学)) / Transcription regulation / Transferase (転移酵素) / Viral nucleoprotein / icosahedral virus / prM-E protein complex structure |