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-Structure paper
タイトル | A refined structure of human aquaporin-1. |
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ジャーナル・号・ページ | FEBS Lett, Vol. 504, Issue 3, Page 206-211, Year 2001 |
掲載日 | 2001年8月31日 |
著者 | B L de Groot / A Engel / H Grubmüller / |
PubMed 要旨 | A refined structure of the human water channel aquaporin-1 is presented. The model rests on the high resolution X-ray structure of the homologous bacterial glycerol transporter GlpF, electron ...A refined structure of the human water channel aquaporin-1 is presented. The model rests on the high resolution X-ray structure of the homologous bacterial glycerol transporter GlpF, electron crystallographic data at 3.8 A resolution and a multiple sequence alignment of the aquaporin superfamily. The crystallographic R and free R values (36.7% and 37.8%) for the refined structure are significantly lower than for previous models. Improved geometry and enhanced stability in molecular dynamics simulations demonstrate a significant improvement of the aquaporin-1 structure. Comparison with previous aquaporin-1 models shows significant differences, not only in the loop regions, but also in the core of the water channel. |
リンク | FEBS Lett / PubMed:11532455 |
手法 | EM (電子線結晶学) |
解像度 | 3.54 Å |
構造データ | PDB-1h6i: |
由来 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / WATER CHANNEL (アクアポリン) / TWO-DIMENSIONAL CRYSTAL |