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-Structure paper
タイトル | Structural studies of two rhinovirus serotypes complexed with fragments of their cellular receptor. |
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ジャーナル・号・ページ | EMBO J, Vol. 18, Issue 22, Page 6249-6259, Year 1999 |
掲載日 | 1999年11月15日 |
著者 | P R Kolatkar / J Bella / N H Olson / C M Bator / T S Baker / M G Rossmann / |
PubMed 要旨 | Two human rhinovirus serotypes complexed with two- and five-domain soluble fragments of the cellular receptor, intercellular adhesion molecule-1, have been investigated by X-ray crystallographic ...Two human rhinovirus serotypes complexed with two- and five-domain soluble fragments of the cellular receptor, intercellular adhesion molecule-1, have been investigated by X-ray crystallographic analyses of the individual components and by cryo-electron microscopy of the complexes. The three-dimensional image reconstructions provide a molecular envelope within which the crystal structures of the viruses and the receptor fragments can be positioned with accuracy. The N-terminal domain of the receptor binds to the rhinovirus 'canyon' surrounding the icosahedral 5-fold axes. Fitting of molecular models into the image reconstruction density identified the residues on the virus that interact with those on the receptor surface, demonstrating complementarity of the electrostatic patterns for the tip of the N-terminal receptor domain and the floor of the canyon. The complexes seen in the image reconstructions probably represent the first stage of a multistep binding process. A mechanism is proposed for the subsequent viral uncoating process. |
リンク | EMBO J / PubMed:10562537 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 3.25 - 28 Å |
構造データ | PDB-1d3e: PDB-1d3i: PDB-1d3l: |
由来 |
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キーワード | Virus/Receptor / HUMAN RHINOVIRUS (ライノウイルス) / HRV16 / ICAM-1 (ICAM-1) / FITTING OF X-RAY STRUCTURES INTO CRYO-EM RECONSTRUCTIONS / COMMON COLD / VIRUS UNCOATING / VIRUS/ VIRAL PROTEIN / RHINOVIRUS-RECEPTOR COMPLEX / Icosahedral virus / Virus-Receptor COMPLEX / HRV14 / CELL ADHESION (細胞接着) / RHINOVIRUS RECEPTOR / ADHESION PROTEIN (接着) / GLYCOPROTEIN (糖タンパク質) / IMMUNOGLOBULIN FOLD |