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-Structure paper
タイトル | Structural basis for energy and electron transfer of the photosystem I-IsiA-flavodoxin supercomplex. |
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ジャーナル・号・ページ | Nat Plants, Vol. 6, Issue 2, Page 167-176, Year 2020 |
掲載日 | 2020年2月10日 |
著者 | Peng Cao / Duanfang Cao / Long Si / Xiaodong Su / Lijin Tian / Wenrui Chang / Zhenfeng Liu / Xinzheng Zhang / Mei Li / |
PubMed 要旨 | Under iron-deficiency stress, which occurs frequently in natural aquatic environments, cyanobacteria reduce the amount of iron-enriched proteins, including photosystem I (PSI) and ferredoxin (Fd), ...Under iron-deficiency stress, which occurs frequently in natural aquatic environments, cyanobacteria reduce the amount of iron-enriched proteins, including photosystem I (PSI) and ferredoxin (Fd), and upregulate the expression of iron-stress-induced proteins A and B (IsiA and flavodoxin (Fld)). Multiple IsiAs function as the peripheral antennae that encircle the PSI core, whereas Fld replaces Fd as the electron receptor of PSI. Here, we report the structures of the PSI-IsiA-Fld and PSI-IsiA supercomplexes from Synechococcus sp. PCC 7942, revealing features that are different from the previously reported PSI structures, and a sophisticated pigment network that involves previously unobserved pigment molecules. Spectroscopic results demonstrated that IsiAs are efficient light harvesters for PSI. Three Flds bind symmetrically to the trimeric PSI core-we reveal the detailed interaction and the electron transport path between PSI and Fld. Our results provide a structural basis for understanding the mechanisms of light harvesting, energy transfer and electron transport of cyanobacterial PSI under stressed conditions. |
リンク | Nat Plants / PubMed:32042157 |
手法 | EM (単粒子) |
解像度 | 2.9 - 3.3 Å |
構造データ | |
化合物 | ChemComp-CLA: ChemComp-PQN: ChemComp-SF4: ChemComp-BCR: ChemComp-LHG: ChemComp-LMU: ChemComp-LMG: ChemComp-SQD: ChemComp-FMN: ChemComp-HOH: |
由来 |
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キーワード | PHOTOSYNTHESIS (光合成) / Photosystem / Antenna / Chlorophyll-binding protein / Membrane protein (膜タンパク質) / iron stress-induced protein A / Flavodoxin (フラボドキシン) |