EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The AAA ATPase Vps4 binds ESCRT-III substrates through a repeating array of dipeptide-binding pockets.
ジャーナル・号・ページ	Elife, Vol. 6, Year 2017
掲載日	2017年11月22日
著者	Han Han / Nicole Monroe / Wesley I Sundquist / Peter S Shen / Christopher P Hill /
PubMed 要旨	The hexameric AAA ATPase Vps4 drives membrane fission by remodeling and disassembling ESCRT-III filaments. Building upon our earlier 4.3 Å resolution cryo-EM structure (Monroe et al., 2017), we now ...The hexameric AAA ATPase Vps4 drives membrane fission by remodeling and disassembling ESCRT-III filaments. Building upon our earlier 4.3 Å resolution cryo-EM structure (Monroe et al., 2017), we now report a 3.2 Å structure of Vps4 bound to an ESCRT-III peptide substrate. The new structure reveals that the peptide approximates a β-strand conformation whose helical symmetry matches that of the five Vps4 subunits it contacts directly. Adjacent Vps4 subunits make equivalent interactions with successive substrate dipeptides through two distinct classes of side chain binding pockets formed primarily by Vps4 pore loop 1. These pockets accommodate a wide range of residues, while main chain hydrogen bonds may help dictate substrate-binding orientation. The structure supports a 'conveyor belt' model of translocation in which ATP binding allows a Vps4 subunit to join the growing end of the helix and engage the substrate, while hydrolysis and release promotes helix disassembly and substrate release at the lagging end.
リンク	Elife / PubMed:29165244 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 6.2 Å
構造データ	8887 6ap1 6bmf EMDB-8887, PDB-6ap1, PDB-6bmf: Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-8888: Unsharpened Map of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-8889: Focused classification map for high position subunit F of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-8890: Focused classification map for low position subunit F of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-8891: Focused classification map for VSL dimer bridging Subunit A and B of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-8892: Focused classification map for VSL dimer bridging Subunit B and C of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-8893: Focused classification map for VSL dimer bridging Subunit C and D of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-8894: Focused classification map for VSL dimer bridging Subunit D and E of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-8895: Focused classification map for VSL dimer bridging Subunit E and F of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 6.2 Å EMDB-8896: Focused classification map for VSL dimer bridging Subunit F and A of Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p 手法: EM (単粒子) / 解像度: 5.2 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母) pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (緑膿菌) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Vps4 / ESCRT (ESCRT) / Vta1 / AAA ATPase