EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and genome release of Twort-like Myoviridae phage with a double-layered baseplate.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 113, Issue 33, Page 9351-9356, Year 2016
掲載日	2016年8月16日
著者	Jiří Nováček / Marta Šiborová / Martin Benešík / Roman Pantůček / Jiří Doškař / Pavel Plevka /
PubMed 要旨	Bacteriophages from the family Myoviridae use double-layered contractile tails to infect bacteria. Contraction of the tail sheath enables the tail tube to penetrate through the bacterial cell wall ...Bacteriophages from the family Myoviridae use double-layered contractile tails to infect bacteria. Contraction of the tail sheath enables the tail tube to penetrate through the bacterial cell wall and serve as a channel for the transport of the phage genome into the cytoplasm. However, the mechanisms controlling the tail contraction and genome release of phages with "double-layered" baseplates were unknown. We used cryo-electron microscopy to show that the binding of the Twort-like phage phi812 to the Staphylococcus aureus cell wall requires a 210° rotation of the heterohexameric receptor-binding and tripod protein complexes within its baseplate about an axis perpendicular to the sixfold axis of the tail. This rotation reorients the receptor-binding proteins to point away from the phage head, and also results in disruption of the interaction of the tripod proteins with the tail sheath, hence triggering its contraction. However, the tail sheath contraction of Myoviridae phages is not sufficient to induce genome ejection. We show that the end of the phi812 double-stranded DNA genome is bound to one protein subunit from a connector complex that also forms an interface between the phage head and tail. The tail sheath contraction induces conformational changes of the neck and connector that result in disruption of the DNA binding. The genome penetrates into the neck, but is stopped at a bottleneck before the tail tube. A subsequent structural change of the tail tube induced by its interaction with the S. aureus cell is required for the genome's release.
リンク	Proc Natl Acad Sci U S A / PubMed:27469164 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.8 - 28.6 Å
構造データ	EMDB-4003: tail tube of phi812-K420 bacteriophage with cotracted tail before genome release 手法: EM (らせん対称) / 解像度: 10.4 Å 4051 5li2 EMDB-4051, PDB-5li2: bacteriophage phi812K1-420 tail sheath and tail tube protein in native tail 手法: EM (らせん対称) / 解像度: 6.2 Å 4052 5li4 EMDB-4052, PDB-5li4: bacteriophage phi812K1-420 tail sheath protein after contraction 手法: EM (らせん対称) / 解像度: 4.2 Å 4053 5lii EMDB-4053, PDB-5lii: bacteriophage phi812K1-420 major capsid protein 手法: EM (単粒子) / 解像度: 3.8 Å 4054 5lij EMDB-4054: bacteriophage phi812K1-420 major capsid protein PDB-5lij: polyalanine chain built in bacteriophage phi812K1-420 cement protein density map 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-8201: Neck and connector complexes of native phi812-K420 bacteriophage, C1 symmetry reconstruction 手法: EM (単粒子) / 解像度: 28.6 Å EMDB-8202: Neck and connector complexes of native phi812-K420 bacteriophage, C5 symmetry reconstruction 手法: EM (単粒子) / 解像度: 27.1 Å EMDB-8203: Neck and connector complexes of native phi812-K420 bacteriophage, C6 symmetry reconstruction 手法: EM (単粒子) / 解像度: 28.3 Å EMDB-8204: Neck and connector complexes of bacteriophage phi812-K420 with contracted tail and full heads, C1 symmetry reconstruction 手法: EM (単粒子) / 解像度: 27.4 Å EMDB-8205: Neck and connector complexes of bacteriophage phi812-K420 with contracted tail and full heads, C5 symmetry reconstruction 手法: EM (単粒子) / 解像度: 28.2 Å EMDB-8206: Neck and connector complexes of bacteriophage phi812-K420 with contracted tail and full heads, C6 symmetry reconstruction 手法: EM (単粒子) / 解像度: 28.2 Å EMDB-8207: Neck and connector complexes of bacteriophage phi812-K420 with contracted tail and empty heads, C1 symmetry reconstruction 手法: EM (単粒子) / 解像度: 27.8 Å EMDB-8208: Neck and connector complexes of bacteriophage phi812-K420 with contracted tail and empty heads, C5 symmetry reconstruction 手法: EM (単粒子) / 解像度: 25.8 Å EMDB-8209: Neck and connector complexes of bacteriophage phi812-K420 with contracted tail and empty heads, C6 symmetry reconstruction 手法: EM (単粒子) / 解像度: 26.7 Å EMDB-8210: Native baseplate of bacteriophage phi812-K420 手法: EM (単粒子) / 解像度: 13.4 Å EMDB-8211: Native baseplate of bacteriophage phi812-K420 with tail fibre in alternative conformation 手法: EM (単粒子) / 解像度: 18.4 Å EMDB-8212: baseplate of bacteriophage phi812-K420 in contracted state 手法: EM (単粒子) / 解像度: 10.8 Å EMDB-8213: receptor-tripod complex of bacteriophage phi812-K420 手法: EM (単粒子) / 解像度: 7.9 Å EMDB-8214: receptor-tripod complex of bacteriophage phi812-K420 手法: EM (単粒子) / 解像度: 10.2 Å EMDB-8304: phi812K1-420 capsid 手法: EM (単粒子) / 解像度: 5.1 Å
由来	staphylococcus phage 812 (ファージ) staphylococcus (ブドウ球菌)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / polyvalent staphylococcal bactoriophage / Myoviridae / tail sheath / tail contraction / VIRUS LIKE PARTICLE (ウイルス様粒子)