EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis.
ジャーナル・号・ページ	Sci Rep, Vol. 8, Issue 1, Page 15701, Year 2018
掲載日	2018年10月24日
著者	Robert N Kirchdoerfer / Nianshuang Wang / Jesper Pallesen / Daniel Wrapp / Hannah L Turner / Christopher A Cottrell / Kizzmekia S Corbett / Barney S Graham / Jason S McLellan / Andrew B Ward /
PubMed 要旨	Severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002 as a highly transmissible pathogenic human betacoronavirus. The viral spike glycoprotein (S) utilizes angiotensin-converting ...Severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002 as a highly transmissible pathogenic human betacoronavirus. The viral spike glycoprotein (S) utilizes angiotensin-converting enzyme 2 (ACE2) as a host protein receptor and mediates fusion of the viral and host membranes, making S essential to viral entry into host cells and host species tropism. As SARS-CoV enters host cells, the viral S is believed to undergo a number of conformational transitions as it is cleaved by host proteases and binds to host receptors. We recently developed stabilizing mutations for coronavirus spikes that prevent the transition from the pre-fusion to post-fusion states. Here, we present cryo-EM analyses of a stabilized trimeric SARS-CoV S, as well as the trypsin-cleaved, stabilized S, and its interactions with ACE2. Neither binding to ACE2 nor cleavage by trypsin at the S1/S2 cleavage site impart large conformational changes within stabilized SARS-CoV S or expose the secondary cleavage site, S2'.
リンク	Sci Rep / PubMed:30356097 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 13.2 Å
構造データ	7573 6crv EMDB-7573, PDB-6crv: SARS Spike Glycoprotein, Stabilized variant, C3 symmetry 手法: EM (単粒子) / 解像度: 3.2 Å 7574 6crw EMDB-7574, PDB-6crw: SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation 手法: EM (単粒子) / 解像度: 3.9 Å 7575 6crx EMDB-7575, PDB-6crx: SARS Spike Glycoprotein, Stabilized variant, two S1 CTDs in the upwards conformation 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-7576: SARS spike glycoprotein, stabilized variant, three S1 CTDs in an upwards conformation 手法: EM (単粒子) / 解像度: 4.5 Å 7577 6crz EMDB-7577, PDB-6crz: SARS Spike Glycoprotein, Trypsin-cleaved, Stabilized variant, C3 symmetry 手法: EM (単粒子) / 解像度: 3.3 Å 7578 6cs0 EMDB-7578, PDB-6cs0: SARS Spike Glycoprotein, Trypsin-cleaved, Stabilized variant, one S1 CTD in an upwards conformation 手法: EM (単粒子) / 解像度: 3.8 Å 7579 6cs1 EMDB-7579, PDB-6cs1: SARS Spike Glycoprotein, Trypsin-cleaved, Stabilized variant, two S1 CTDs in an upwards conformation 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-7580: SARS spike glycoprotein, trypsin-cleaved, stabilized variant, three S1 CTDs in an upwards conformation 手法: EM (単粒子) / 解像度: 10.6 Å EMDB-7581: SARS spike glycoprotein, trypsin-cleaved, stabilized variant, three S1 CTDs in a downwards conformation 手法: EM (単粒子) / 解像度: 5.3 Å 7582 6cs2 EMDB-7582, PDB-6cs2: SARS Spike Glycoprotein - human ACE2 complex, Stabilized variant, all ACE2-bound particles 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-7584: SARS spike glycoprotein, stabilized variant, ACE2-bound dataset, S1 CTD configuration: upwards, downwards, downwards 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-7585: SARS spike glycoprotein, stabilized variant, ACE2-bound dataset, S1 CTD configuration: upwards, upwards, downwards 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-7586: SARS spike glycoprotein, stabilized variant, S1 CTD configuration: ACE2-bound, downwards, downwards 手法: EM (単粒子) / 解像度: 6.2 Å EMDB-7601: SARS spike glycoprotein, stabilized variant, S1 CTD configuration: ACE2-bound, downwards, upwards 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-7602: SARS spike glycoprotein, stabilized variant, S1 CTD configuration: ACE2-bound, upwards, downwards 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-7603: SARS spike glycoprotein, stabilized variant, S1 CTD configurations: ACE2-bound, upwards, upwards 手法: EM (単粒子) / 解像度: 13.2 Å EMDB-7604: SARS spike glycoprotein, stabilized variant, S1 CTD configurations: ACE2-bound, ACE2-bound, downwards 手法: EM (単粒子) / 解像度: 8.1 Å EMDB-7605: SARS spike glycoprotein, stabilized variant, S1 CTD configurations: ACE2-bound, ACE2-bound, upwards 手法: EM (単粒子) / 解像度: 8.8 Å EMDB-7606: SARS spike glycoprotein, stabilized variant, S1 CTD configurations: ACE2-bound, ACE2-bound, ACE2-bound 手法: EM (単粒子) / 解像度: 9.3 Å EMDB-7607: SARS spike glycoprotein, stabilized variant, S1 CTD configurations: ACE2-bound, upwards/downwards mix, downwards 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-7608: SARS spike glycoprotein, focused refinement of S1 CTD bound to ACE2 手法: EM (単粒子) / 解像度: 7.9 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	SARS coronavirus (SARS コロナウイルス) enterobacteria phage t4 (ファージ) homo sapiens (ヒト) human sars coronavirus (SARSコロナウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / membrane fusion / glycoprotein (糖タンパク質) / receptor binding (受容体) / viral protein/hydrolase (ウイルス性) / viral protein-hydrolase complex (ウイルス性)