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-Structure paper
タイトル | Structure of the voltage-gated calcium channel Cav1.1 complex. |
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ジャーナル・号・ページ | Science, Vol. 350, Issue 6267, Page aad2395, Year 2015 |
掲載日 | 2015年12月18日 |
著者 | Jianping Wu / Zhen Yan / Zhangqiang Li / Chuangye Yan / Shan Lu / Mengqiu Dong / Nieng Yan / |
PubMed 要旨 | The voltage-gated calcium channel Ca(v)1.1 is engaged in the excitation-contraction coupling of skeletal muscles. The Ca(v)1.1 complex consists of the pore-forming subunit α1 and auxiliary subunits ...The voltage-gated calcium channel Ca(v)1.1 is engaged in the excitation-contraction coupling of skeletal muscles. The Ca(v)1.1 complex consists of the pore-forming subunit α1 and auxiliary subunits α2δ, β, and γ. We report the structure of the rabbit Ca(v)1.1 complex determined by single-particle cryo-electron microscopy. The four homologous repeats of the α1 subunit are arranged clockwise in the extracellular view. The γ subunit, whose structure resembles claudins, interacts with the voltage-sensing domain of repeat IV (VSD(IV)), whereas the cytosolic β subunit is located adjacent to VSD(II) of α1. The α2 subunit interacts with the extracellular loops of repeats I to III through its VWA and Cache1 domains. The structure reveals the architecture of a prototypical eukaryotic Ca(v) channel and provides a framework for understanding the function and disease mechanisms of Ca(v) and Na(v) channels. |
リンク | Science / PubMed:26680202 |
手法 | EM (単粒子) |
解像度 | 4.2 - 6.1 Å |
構造データ | |
化合物 | ChemComp-CA: ChemComp-NAG: ChemComp-BMA: |
由来 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / voltage-gated calcium channel (電位依存性カルシウムチャネル) |