EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Distinct conformational spectrum of homologous multidrug ABC transporters.
ジャーナル・号・ページ	Structure, Vol. 23, Issue 3, Page 450-460, Year 2015
掲載日	2015年3月3日
著者	Arne Moeller / Sung Chang Lee / Houchao Tao / Jeffrey A Speir / Geoffrey Chang / Ina L Urbatsch / Clinton S Potter / Bridget Carragher / Qinghai Zhang /
PubMed 要旨	ATP-binding cassette (ABC) exporters are ubiquitously found in all kingdoms of life and their members play significant roles in mediating drug pharmacokinetics and multidrug resistance in the clinic. ...ATP-binding cassette (ABC) exporters are ubiquitously found in all kingdoms of life and their members play significant roles in mediating drug pharmacokinetics and multidrug resistance in the clinic. Significant questions and controversies remain regarding the relevance of their conformations observed in X-ray structures, their structural dynamics, and mechanism of transport. Here, we used single particle electron microscopy (EM) to delineate the entire conformational spectrum of two homologous ABC exporters (bacterial MsbA and mammalian P-glycoprotein) and the influence of nucleotide and substrate binding. Newly developed amphiphiles in complex with lipids that support high protein stability and activity enabled EM visualization of individual complexes in a membrane-mimicking environment. The data provide a comprehensive view of the conformational flexibility of these ABC exporters under various states and demonstrate not only similarities but striking differences between their mechanistic and energetic regulation of conformational changes.
リンク	Structure / PubMed:25661651 / PubMed Central
手法	EM (単粒子)
解像度	30.0 - 40.0 Å
構造データ	EMDB-6007: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 40.0 Å EMDB-6018: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6019: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 40.0 Å EMDB-6020: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6021: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6022: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6023: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6024: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6025: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6026: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6027: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6028: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6029: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6030: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6031: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6032: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-6033: Conformational Spectrum of Multidrug ABC Transporters Revealed by Single Particle Electron Microscopy 手法: EM (単粒子) / 解像度: 30.0 Å
由来	Mus musculus (ハツカネズミ) Escherichia coli (大腸菌)