EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Escherichia coli rimM and yjeQ null strains accumulate immature 30S subunits of similar structure and protein complement.
ジャーナル・号・ページ	RNA, Vol. 19, Issue 6, Page 789-802, Year 2013
掲載日	2013年4月23日
著者	Vivian Leong / Meredith Kent / Ahmad Jomaa / Joaquin Ortega /
PubMed 要旨	Assembly of the Escherichia coli 30S ribosomal subunits proceeds through multiple parallel pathways. The protein factors RimM, YjeQ, RbfA, and Era work in conjunction to assist at the late stages of ...Assembly of the Escherichia coli 30S ribosomal subunits proceeds through multiple parallel pathways. The protein factors RimM, YjeQ, RbfA, and Era work in conjunction to assist at the late stages of the maturation process of the small subunit. However, it is unclear how the functional interplay between these factors occurs in the context of multiple parallel pathways. To understand how these factors work together, we have characterized the immature 30S subunits that accumulate in ΔrimM cells and compared them with immature 30S subunits from a ΔyjeQ strain. The cryo-EM maps obtained from these particles showed that the densities representing helices 44 and 45 in the rRNA were partially missing, suggesting mobility of these motifs. These 30S subunits were also partially depleted in all tertiary ribosomal proteins, particularly those binding in the head domain. Using image classification, we identified four subpopulations of ΔrimM immature 30S subunits differing in the amount of missing density for helices 44 and 45, as well as the amount of density existing in these maps for the underrepresented proteins. The structural defects found in these immature subunits resembled those of the 30S subunits that accumulate in the ΔyjeQ strain. These findings are consistent with an "early convergency model" in which multiple parallel assembly pathways of the 30S subunit converge into a late assembly intermediate, as opposed to the mature state. Functionally related factors will bind to this intermediate to catalyze the last steps of maturation leading to the mature 30S subunit.
リンク	RNA / PubMed:23611982 / PubMed Central
手法	EM (単粒子)
解像度	17.0 Å
構造データ	EMDB-5595: Structure of the immature 30S subunit from a Escherichia coli rimM null strain. Conformation 1. 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-5596: Structure of the immature 30S subunit from a Escherichia coli rimM null strain. Conformation 2. 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-5597: Structure of the immature 30S subunit from a Escherichia coli rimM null strain. Conformation 3. 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-5598: Structure of the immature 30S subunit from a Escherichia coli rimM null strain. Conformation 4. 手法: EM (単粒子) / 解像度: 17.0 Å
由来	Escherichia coli (大腸菌)