EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 107, Issue 32, Page 14111-14115, Year 2010
掲載日	2010年8月10日
著者	Tsutomu Matsui / Gabriel C Lander / Reza Khayat / John E Johnson /
PubMed 要旨	Effective antiviral agents are difficult to develop because of the close relationship between the cell biology of the virus and host. However, viral capsid maturation, the in vivo process where the ...Effective antiviral agents are difficult to develop because of the close relationship between the cell biology of the virus and host. However, viral capsid maturation, the in vivo process where the particle transitions from a noninfectious provirion to an infectious virion, is an ideal process to interrupt because the provirion is usually fragile and the conversion to the virion often involves large conformational changes and autocatalytic chemistry that can be hampered by small molecules. The Nudaurelia capensis omega virus (N omegaV) is one of the few eukaryotic viruses where this process can be investigated in vitro with a variety of biophysical methods, allowing fundamental chemical and structural principles of the maturation to be established. It has a T = 4 quasi-equivalent capsid with a dramatic maturation pathway that includes a particle size reduction of 100 A and an autocatalytic cleavage. Here we use cryo-EM and difference maps, computed at three time points following maturation initiation, to show that regions of N omegaV subunit folding are maturation dependent and occur at rates determined by their quasi-equivalent position in the capsid, explaining the unusual kinetics of the maturation cleavage. This study shows that folding is rapid and peptide chain self-cleavage occurs early for subunits adjacent to 3-fold and 5-fold icosahedral symmetry elements and that folding is slower in regions where molecular switches are required for the formation of the proper interfacial contacts. The results connect viral maturation to the well-studied assembly-dependent folding that occurs in the formation of cellular complexes.
リンク	Proc Natl Acad Sci U S A / PubMed:20660783 / PubMed Central
手法	EM (単粒子)
解像度	8.3 - 9.8 Å
構造データ	EMDB-5425: Cryo-EM reconstruction of fully-mature capsid of Nudaurelia capensis omega virus (NwV) 手法: EM (単粒子) / 解像度: 9.8 Å EMDB-5426: Cryo-EM reconstruction of Nudaurelia capensis omega virus (NwV) capsid at 3min time point following maturation initiation 手法: EM (単粒子) / 解像度: 9.3 Å EMDB-5427: Cryo-EM reconstruction of Nudaurelia capensis omega virus (NwV) capsid at 30min time point following maturation initiation 手法: EM (単粒子) / 解像度: 8.6 Å EMDB-5428: Cryo-EM reconstruction of Nudaurelia capensis omega virus (NwV) capsid at 4hr time point following maturation initiation 手法: EM (単粒子) / 解像度: 8.3 Å