EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The role of tubulin-tubulin lattice contacts in the mechanism of microtubule dynamic instability.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 25, Issue 7, Page 607-615, Year 2018
掲載日	2018年7月2日
著者	Szymon W Manka / Carolyn A Moores /
PubMed 要旨	Microtubules form from longitudinally and laterally assembling tubulin α-β dimers. The assembly induces strain in tubulin, resulting in cycles of microtubule catastrophe and regrowth. This 'dynamic ...Microtubules form from longitudinally and laterally assembling tubulin α-β dimers. The assembly induces strain in tubulin, resulting in cycles of microtubule catastrophe and regrowth. This 'dynamic instability' is governed by GTP hydrolysis that renders the microtubule lattice unstable, but it is unclear how. We used a human microtubule nucleating and stabilizing neuronal protein, doublecortin, and high-resolution cryo-EM to capture tubulin's elusive hydrolysis intermediate GDP•Pi state, alongside the prehydrolysis analog GMPCPP state and the posthydrolysis GDP state with and without an anticancer drug, Taxol. GTP hydrolysis to GDP•Pi followed by Pi release constitutes two distinct structural transitions, causing unevenly distributed compressions of tubulin dimers, thereby tightening longitudinal and loosening lateral interdimer contacts. We conclude that microtubule catastrophe is triggered because the lateral contacts can no longer counteract the strain energy stored in the lattice, while reinforcement of the longitudinal contacts may support generation of force.
リンク	Nat Struct Mol Biol / PubMed:29967541 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 4.4 Å
構造データ	3961 6evw EMDB-3961, PDB-6evw: Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin 手法: EM (単粒子) / 解像度: 4.4 Å 3962 6evx EMDB-3962, PDB-6evx: Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin 手法: EM (単粒子) / 解像度: 4.2 Å 3963 6evy EMDB-3963, PDB-6evy: Cryo-EM structure of GTPgammaS-microtubule co-polymerised with doublecortin 手法: EM (単粒子) / 解像度: 4.4 Å 3964 6evz EMDB-3964, PDB-6evz: Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin 手法: EM (単粒子) / 解像度: 3.8 Å 3965 6ew0 EMDB-3965: Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin PDB-6ew0: Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin and supplemented with Taxol 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S ChemComp-TA1: TAXOL / パクリタキセル / 薬剤, 化学療法薬YM / パクリタキセル
由来	sus sucrofa (ブタ) sus scrofa (ブタ)
キーワード	STRUCTURAL PROTEIN (タンパク質) / microtubule (微小管) / GTPase (GTPアーゼ) / tubulin (チューブリン) / Taxol (パクリタキセル)