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-Structure paper
タイトル | Molecular basis of A. thaliana KEOPS complex in biosynthesizing tRNA t6A. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 52, Issue 8, Page 4523-4540, Year 2024 |
掲載日 | 2024年5月8日 |
著者 | Xinxing Zheng / Chenchen Su / Lei Duan / Mengqi Jin / Yongtao Sun / Li Zhu / Wenhua Zhang / |
PubMed 要旨 | In archaea and eukaryotes, the evolutionarily conserved KEOPS is composed of four core subunits-Kae1, Bud32, Cgi121 and Pcc1, and a fifth Gon7/Pcc2 that is found in fungi and metazoa. KEOPS ...In archaea and eukaryotes, the evolutionarily conserved KEOPS is composed of four core subunits-Kae1, Bud32, Cgi121 and Pcc1, and a fifth Gon7/Pcc2 that is found in fungi and metazoa. KEOPS cooperates with Sua5/YRDC to catalyze the biosynthesis of tRNA N6-threonylcarbamoyladenosine (t6A), an essential modification needed for fitness of cellular organisms. Biochemical and structural characterizations of KEOPSs from archaea, yeast and humans have determined a t6A-catalytic role for Kae1 and auxiliary roles for other subunits. However, the precise molecular workings of KEOPSs still remain poorly understood. Here, we investigated the biochemical functions of A. thaliana KEOPS and determined a cryo-EM structure of A. thaliana KEOPS dimer. We show that A. thaliana KEOPS is composed of KAE1, BUD32, CGI121 and PCC1, which adopts a conserved overall arrangement. PCC1 dimerization leads to a KEOPS dimer that is needed for an active t6A-catalytic KEOPS-tRNA assembly. BUD32 participates in direct binding of tRNA to KEOPS and modulates the t6A-catalytic activity of KEOPS via its C-terminal tail and ATP to ADP hydrolysis. CGI121 promotes the binding of tRNA to KEOPS and potentiates the t6A-catalytic activity of KEOPS. These data and findings provide insights into mechanistic understanding of KEOPS machineries. |
リンク | Nucleic Acids Res / PubMed:38477398 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.7 Å |
構造データ | EMDB-36808, PDB-8k20: |
化合物 | ChemComp-FE: |
由来 |
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キーワード | TRANSFERASE (転移酵素) / KEOPS complex / tRNA t6A synthase / RNA-binding protein (RNA結合タンパク質) |