EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A 5+1 assemble-to-activate mechanism of the Lon proteolytic machine.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 7340, Year 2023
掲載日	2023年11月13日
著者	Shanshan Li / Kan-Yen Hsieh / Chiao-I Kuo / Tzu-Chi Lin / Szu-Hui Lee / Yi-Ru Chen / Chun-Hsiung Wang / Meng-Ru Ho / See-Yeun Ting / Kaiming Zhang / Chung-I Chang /
PubMed 要旨	Many AAA+ (ATPases associated with diverse cellular activities) proteins function as protein or DNA remodelers by threading the substrate through the central pore of their hexameric assemblies. In ...Many AAA+ (ATPases associated with diverse cellular activities) proteins function as protein or DNA remodelers by threading the substrate through the central pore of their hexameric assemblies. In this ATP-dependent translocating state, the substrate is gripped by the pore loops of the ATPase domains arranged in a universal right-handed spiral staircase organization. However, the process by which a AAA+ protein is activated to adopt this substrate-pore-loop arrangement remains unknown. We show here, using cryo-electron microscopy (cryo-EM), that the activation process of the Lon AAA+ protease may involve a pentameric assembly and a substrate-dependent incorporation of the sixth protomer to form the substrate-pore-loop contacts seen in the translocating state. Based on the structural results, we design truncated monomeric mutants that inhibit Lon activity by binding to the native pentamer and demonstrated that expressing these monomeric mutants in Escherichia coli cells containing functional Lon elicits specific phenotypes associated with lon deficiency, including the inhibition of persister cell formation. These findings uncover a substrate-dependent assembly process for the activation of a AAA+ protein and demonstrate a targeted approach to selectively inhibit its function within cells.
リンク	Nat Commun / PubMed:37957149 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 5.8 Å
構造データ	34000 7yph EMDB-34000, PDB-7yph: Open-spiral pentamer of the substrate-free Lon protease with a Y224S mutation 手法: EM (単粒子) / 解像度: 3.68 Å 34001 7ypi EMDB-34001, PDB-7ypi: Spiral hexamer of the substrate-free Lon protease with a Y224S mutation 手法: EM (単粒子) / 解像度: 3.8 Å 34002 7ypj EMDB-34002, PDB-7ypj: Spiral pentamer of the substrate-free Lon protease with a S678A mutation 手法: EM (単粒子) / 解像度: 3.8 Å 34003 7ypk EMDB-34003, PDB-7ypk: Close-ring hexamer of the substrate-bound Lon protease with an S678A mutation 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-34004: Spiral hexamer of the substrate-free Lon protease with an S678A mutation 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-34005: Open-spiral pentamer of the substrate-free Lon protease with Y397A and S678A mutations 手法: EM (単粒子) / 解像度: 3.72 Å EMDB-34006: Spiral hexamer of the substrate-free Lon protease with Y397A and S678A mutations 手法: EM (単粒子) / 解像度: 4.08 Å 34107 7yuh EMDB-34107, PDB-7yuh: MtaLon-Apo for the spiral oligomers of trimer 手法: EM (単粒子) / 解像度: 3.7 Å 34108 7yum EMDB-34108, PDB-7yum: MtaLon-Apo for the spiral oligomers of tetramer 手法: EM (単粒子) / 解像度: 3.8 Å 34109 7yup EMDB-34109, PDB-7yup: MtaLon-Apo for the spiral oligomers of pentamer 手法: EM (単粒子) / 解像度: 3.7 Å 34110 7yut EMDB-34110, PDB-7yut: MtaLon-Apo for the spiral oligomers of hexamer 手法: EM (単粒子) / 解像度: 4.1 Å 34111 7yuu EMDB-34111, PDB-7yuu: MtaLon-ADP for the spiral oligomers of trimer 手法: EM (単粒子) / 解像度: 5.8 Å 34112 7yuv EMDB-34112, PDB-7yuv: MtaLon-ADP for the spiral oligomers of tetramer 手法: EM (単粒子) / 解像度: 3.8 Å 34113 7yuw EMDB-34113, PDB-7yuw: MtaLon-ADP for the spiral oligomers of pentamer 手法: EM (単粒子) / 解像度: 3.6 Å 34114 7yux EMDB-34114, PDB-7yux: MtaLon-ADP for the spiral oligomers of hexamer 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-34116: Spiral pentamer of the substrate-free Lon protease with an M217A mutation 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-34117: Spiral hexamer of the substrate-free Lon protease with an M217A mutation 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-36865: Spiral pentameric form of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing an E613K mutation 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-36866: Spiral hexameric form of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing an E613K mutation 手法: EM (単粒子) / 解像度: 4.3 Å 36867 8k3y EMDB-36867, PDB-8k3y: The "5+1" heteromeric structure of Lon protease consisting of a spiral pentamer with Y224S mutation and an N-terminal-truncated monomeric E613K mutant 手法: EM (単粒子) / 解像度: 4.42 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	meiothermus taiwanensis (バクテリア) bos taurus (ウシ)
キーワード	HYDROLASE (加水分解酵素) / Lon protease / hydrolysis (加水分解) / AAA proteins / AAA / protease (プロテアーゼ) / complex / proteolysis (タンパク質分解) / Assembly / AAA+ protein / ATPase (ATPアーゼ)