EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Asymmetric pendrin homodimer reveals its molecular mechanism as anion exchanger.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 3012, Year 2023
掲載日	2023年5月25日
著者	Qianying Liu / Xiang Zhang / Hui Huang / Yuxin Chen / Fang Wang / Aihua Hao / Wuqiang Zhan / Qiyu Mao / Yuxia Hu / Lin Han / Yifang Sun / Meng Zhang / Zhimin Liu / Geng-Lin Li / Weijia Zhang / Yilai Shu / Lei Sun / Zhenguo Chen /
PubMed 要旨	Pendrin (SLC26A4) is an anion exchanger expressed in the apical membranes of selected epithelia. Pendrin ablation causes Pendred syndrome, a genetic disorder associated with sensorineural hearing ...Pendrin (SLC26A4) is an anion exchanger expressed in the apical membranes of selected epithelia. Pendrin ablation causes Pendred syndrome, a genetic disorder associated with sensorineural hearing loss, hypothyroid goiter, and reduced blood pressure. However its molecular structure has remained unknown, limiting our understanding of the structural basis of transport. Here, we determine the cryo-electron microscopy structures of mouse pendrin with symmetric and asymmetric homodimer conformations. The asymmetric homodimer consists of one inward-facing protomer and the other outward-facing protomer, representing coincident uptake and secretion- a unique state of pendrin as an electroneutral exchanger. The multiple conformations presented here provide an inverted alternate-access mechanism for anion exchange. The structural and functional data presented here disclose the properties of an anion exchange cleft and help understand the importance of disease-associated variants, which will shed light on the pendrin exchange mechanism.
リンク	Nat Commun / PubMed:37230976 / PubMed Central
手法	EM (単粒子)
解像度	3.25 - 4.1 Å
構造データ	32555 7wk1 EMDB-32555, PDB-7wk1: Mouse Pendrin bound chloride in inward state 手法: EM (単粒子) / 解像度: 3.25 Å 32561 7wk7 EMDB-32561, PDB-7wk7: Mouse Pendrin bound bicarbonate in inward state 手法: EM (単粒子) / 解像度: 3.49 Å 32574 7wl2 EMDB-32574: mouse Pendrin in bicarbonate and iodide buffer in inward state PDB-7wl2: Mouse Pendrin in bicarbonate and iodide buffer in inward state 手法: EM (単粒子) / 解像度: 3.25 Å 32576 7wl7 EMDB-32576, PDB-7wl7: Mouse Pendrin in chloride and bicarbonate buffer in inward state 手法: EM (単粒子) / 解像度: 3.51 Å 32577 7wl8 EMDB-32577, PDB-7wl8: Mouse Pendrin in chloride and iodide buffer in inward state 手法: EM (単粒子) / 解像度: 3.4 Å 32578 7wl9 EMDB-32578, PDB-7wl9: Mouse Pendrin in chloride and bicarbonate in asymmetric state 手法: EM (単粒子) / 解像度: 3.78 Å 32579 7wla EMDB-32579, PDB-7wla: Mouse Pendrin in bicarbonate and iodide buffer in asymmetric state 手法: EM (単粒子) / 解像度: 3.76 Å 32580 7wlb EMDB-32580, PDB-7wlb: Mouse Pendrin in chloride and iodide buffer in asymmetric state 手法: EM (単粒子) / 解像度: 4.1 Å 32583 7wle EMDB-32583, PDB-7wle: Mouse Pendrin in chloride and bicarbonate buffer in outward state 手法: EM (単粒子) / 解像度: 3.62 Å EMDB-33232: Local refinement of outward-open TM domain of mouse Pendrin in chloride and bicarbonate in asymmetric state 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-BCT: BICARBONATE ION / 炭酸水素アニオン / pH緩衝剤*YM / 炭酸水素塩
由来	mus musculus (ハツカネズミ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / exchange / transport / slc