EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and mechanism of the Rubisco-assembly chaperone Raf1.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 22, Issue 9, Page 720-728, Year 2015
掲載日	2015年8月3日
著者	Thomas Hauser / Javaid Y Bhat / Goran Miličić / Petra Wendler / F Ulrich Hartl / Andreas Bracher / Manajit Hayer-Hartl /
PubMed 要旨	Biogenesis of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits, requires assembly chaperones. Here we analyzed the role of Rubisco accumulation ...Biogenesis of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits, requires assembly chaperones. Here we analyzed the role of Rubisco accumulation factor1 (Raf1), a dimer of ∼40-kDa subunits. We find that Raf1 from Synechococcus elongatus acts downstream of chaperonin-assisted RbcL folding by stabilizing RbcL antiparallel dimers for assembly into RbcL8 complexes with four Raf1 dimers bound. Raf1 displacement by RbcS results in holoenzyme formation. Crystal structures show that Raf1 from Arabidopsis thaliana consists of a β-sheet dimerization domain and a flexibly linked α-helical domain. Chemical cross-linking and EM reconstruction indicate that the β-domains bind along the equator of each RbcL2 unit, and the α-helical domains embrace the top and bottom edges of RbcL2. Raf1 fulfills a role similar to that of the assembly chaperone RbcX, thus suggesting that functionally redundant factors ensure efficient Rubisco biogenesis.
リンク	Nat Struct Mol Biol / PubMed:26237510
手法	EM (単粒子) / X線回折
解像度	1.954 - 24.8 Å
構造データ	EMDB-3051: Structure and mechanism of the Rubisco assembly chaperone Raf1 手法: EM (単粒子) / 解像度: 24.8 Å EMDB-3052: Structure and mechanism of the Rubisco assembly chaperone Raf1 手法: EM (単粒子) / 解像度: 21.4 Å EMDB-3053: Structure and mechanism of the Rubisco assembly chaperone Raf1 手法: EM (単粒子) / 解像度: 24.6 Å PDB-4wt3: The N-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana 手法: X-RAY DIFFRACTION / 解像度: 1.954 Å PDB-4wt4: The C-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana, crystal form I 手法: X-RAY DIFFRACTION / 解像度: 2.81 Å PDB-4wt5: The C-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana, crystal form II 手法: X-RAY DIFFRACTION / 解像度: 2.568 Å
化合物	ChemComp-HOH: WATER / 水 ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩
由来	Thermosynechococcus elongatus BP-1 (バクテリア) Synechococcus elongatus PCC 6301 (バクテリア) arabidopsis thaliana (シロイヌナズナ)
キーワード	CHAPERONE (シャペロン) / assembly chaperone