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-Structure paper
タイトル | Molecular mechanism for Tn7-like transposon recruitment by a type I-B CRISPR effector. |
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ジャーナル・号・ページ | Cell, Vol. 186, Issue 19, Page 4204-4215.e19, Year 2023 |
掲載日 | 2023年9月14日 |
著者 | Shukun Wang / Clinton Gabel / Romana Siddique / Thomas Klose / Leifu Chang / |
PubMed 要旨 | Tn7-like transposons have co-opted CRISPR-Cas systems to facilitate the movement of their own DNA. These CRISPR-associated transposons (CASTs) are promising tools for programmable gene knockin. A key ...Tn7-like transposons have co-opted CRISPR-Cas systems to facilitate the movement of their own DNA. These CRISPR-associated transposons (CASTs) are promising tools for programmable gene knockin. A key feature of CASTs is their ability to recruit Tn7-like transposons to nuclease-deficient CRISPR effectors. However, how Tn7-like transposons are recruited by diverse CRISPR effectors remains poorly understood. Here, we present the cryo-EM structure of a recruitment complex comprising the Cascade complex, TniQ, TnsC, and the target DNA in the type I-B CAST from Peltigera membranacea cyanobiont 210A. Target DNA recognition by Cascade induces conformational changes in Cas6 and primes TniQ recruitment through its C-terminal domain. The N-terminal domain of TniQ is bound to the seam region of the TnsC spiral heptamer. Our findings provide insights into the diverse mechanisms for the recruitment of Tn7-like transposons to CRISPR effectors and will aid in the development of CASTs as gene knockin tools. |
リンク | Cell / PubMed:37557170 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.83 - 3.65 Å |
構造データ | EMDB-28980, PDB-8fcj: EMDB-28993, PDB-8fcu: EMDB-28994, PDB-8fcv: EMDB-28997, PDB-8fcw: EMDB-28998, PDB-8fcx: EMDB-29000, PDB-8fd2: EMDB-29001, PDB-8fd3: EMDB-29039, PDB-8ff4: EMDB-29040, PDB-8ff5: |
化合物 | ChemComp-ATP: ChemComp-MG: |
由来 |
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キーワード | DNA BINDING PROTEIN (DNA結合タンパク質) / CRISPR (CRISPR) / DNA recognition (DNA型鑑定) / TnsC / TniQ / Transposon (トランスポゾン) / DNA (デオキシリボ核酸) / CAST / Cascade / I-B |