+検索条件
-Structure paper
タイトル | Ribosome. The structure of the human mitochondrial ribosome. |
---|---|
ジャーナル・号・ページ | Science, Vol. 348, Issue 6230, Page 95-98, Year 2015 |
掲載日 | 2015年4月3日 |
著者 | Alexey Amunts / Alan Brown / Jaan Toots / Sjors H W Scheres / V Ramakrishnan / |
PubMed 要旨 | The highly divergent ribosomes of human mitochondria (mitoribosomes) synthesize 13 essential proteins of oxidative phosphorylation complexes. We have determined the structure of the intact ...The highly divergent ribosomes of human mitochondria (mitoribosomes) synthesize 13 essential proteins of oxidative phosphorylation complexes. We have determined the structure of the intact mitoribosome to 3.5 angstrom resolution by means of single-particle electron cryogenic microscopy. It reveals 80 extensively interconnected proteins, 36 of which are specific to mitochondria, and three ribosomal RNA molecules. The head domain of the small subunit, particularly the messenger (mRNA) channel, is highly remodeled. Many intersubunit bridges are specific to the mitoribosome, which adopts conformations involving ratcheting or rolling of the small subunit that are distinct from those seen in bacteria or eukaryotes. An intrinsic guanosine triphosphatase mediates a contact between the head and central protuberance. The structure provides a reference for analysis of mutations that cause severe pathologies and for future drug design. |
リンク | Science / PubMed:25838379 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.27 - 4.7 Å |
構造データ | EMDB-2876: The structure of the human mitochondrial ribosome (class 1). EMDB-2877: EMDB-2878: EMDB-2879: EMDB-2880: EMDB-2881: |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-GDP: |
由来 |
|
キーワード | RIBOSOME (リボソーム) / Mitochondria (ミトコンドリア) / translation (翻訳 (生物学)) |