EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Three-dimensional electron microscopy reconstruction and cysteine-mediated crosslinking provide a model of the type III secretion system needle tip complex.
ジャーナル・号・ページ	Mol Microbiol, Vol. 95, Issue 1, Page 31-50, Year 2015
掲載日	2014年11月27日
著者	Martin Cheung / Da-Kang Shen / Fumiaki Makino / Takayuki Kato / A Dorothea Roehrich / Isabel Martinez-Argudo / Matthew L Walker / Isabel Murillo / Xia Liu / Maria Pain / James Brown / Gordon Frazer / Judith Mantell / Petros Mina / Thomas Todd / Richard B Sessions / Keiichi Namba / Ariel J Blocker /
PubMed 要旨	Type III secretion systems are found in many Gram-negative bacteria. They are activated by contact with eukaryotic cells and inject virulence proteins inside them. Host cell detection requires a ...Type III secretion systems are found in many Gram-negative bacteria. They are activated by contact with eukaryotic cells and inject virulence proteins inside them. Host cell detection requires a protein complex located at the tip of the device's external injection needle. The Shigella tip complex (TC) is composed of IpaD, a hydrophilic protein, and IpaB, a hydrophobic protein, which later forms part of the injection pore in the host membrane. Here we used labelling and crosslinking methods to show that TCs from a ΔipaB strain contain five IpaD subunits while the TCs from wild-type can also contain one IpaB and four IpaD subunits. Electron microscopy followed by single particle and helical image analysis was used to reconstruct three-dimensional images of TCs at ∼ 20 Å resolution. Docking of an IpaD crystal structure, constrained by the crosslinks observed, reveals that TC organisation is different from that of all previously proposed models. Our findings suggest new mechanisms for TC assembly and function. The TC is the only site within these secretion systems targeted by disease-protecting antibodies. By suggesting how these act, our work will allow improvement of prophylactic and therapeutic strategies.
リンク	Mol Microbiol / PubMed:25353930 / PubMed Central
手法	EM (単粒子)
解像度	19.0 - 24.0 Å
構造データ	2801 4d3e EMDB-2801: Negative stain electron microscopy reconstruction of the wild type tip complex from the type III secretion system of Shigella flexneri PDB-4d3e: Tetramer of IpaD, modified from 2J0O, fitted into negative stain electron microscopy reconstruction of the wild type tip complex from the type III secretion system of Shigella flexneri 手法: EM (単粒子) / 解像度: 24.0 Å EMDB-2802: Negative stain electron microscopy reconstruction of the tip complex from the type III secretion system of Shigella flexneri lacking IpaB 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-2803: Negative stain electron microscopy reconstruction of the tip complex and needle from the type III secretion system of Shigella flexneri with MxiH mutation P44A 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-2804: Negative stain electron microscopy reconstruction of the tip complex from the type III secretion system of Shigella flexneri with MxiH mutation Q51A 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-2805: Refined negative stain electron microscopy reconstruction of the tip complex from the type III secretion system of Shigella flexneri with MxiH mutation Q51A 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-2806: Negative stain electron microscopy reconstruction of the tip complex from the type III secretion system of Shigella flexneri with MxiH mutations P44A+Q51A 手法: EM (単粒子) / 解像度: 24.0 Å
由来	Shigella flexneri (フレクスナー赤痢菌) shigella flexneri 5a str. m90t (フレクスナー赤痢菌)
キーワード	CELL INVASION / TIP COMPLEX / TYPE III SECRETION SYSTEM / SHIGELLA FLEXNERI / WILD TYPE (野生型) / IPAD