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-Structure paper
タイトル | Structural evolution of glycan recognition by a family of potent HIV antibodies. |
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ジャーナル・号・ページ | Cell, Vol. 159, Issue 1, Page 69-79, Year 2014 |
掲載日 | 2014年9月25日 |
著者 | Fernando Garces / Devin Sok / Leopold Kong / Ryan McBride / Helen J Kim / Karen F Saye-Francisco / Jean-Philippe Julien / Yuanzi Hua / Albert Cupo / John P Moore / James C Paulson / Andrew B Ward / Dennis R Burton / Ian A Wilson / |
PubMed 要旨 | The HIV envelope glycoprotein (Env) is densely covered with self-glycans that should help shield it from recognition by the human immune system. Here, we examine how a particularly potent family of ...The HIV envelope glycoprotein (Env) is densely covered with self-glycans that should help shield it from recognition by the human immune system. Here, we examine how a particularly potent family of broadly neutralizing antibodies (Abs) has evolved common and distinct structural features to counter the glycan shield and interact with both glycan and protein components of HIV Env. The inferred germline antibody already harbors potential binding pockets for a glycan and a short protein segment. Affinity maturation then leads to divergent evolutionary branches that either focus on a single glycan and protein segment (e.g., Ab PGT124) or engage multiple glycans (e.g., Abs PGT121-123). Furthermore, other surrounding glycans are avoided by selecting an appropriate initial antibody shape that prevents steric hindrance. Such molecular recognition lessons are important for engineering proteins that can recognize or accommodate glycans. |
リンク | Cell / PubMed:25259921 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.4969 - 17.8 Å |
構造データ | EMDB-2753: PDB-4r26: PDB-4r2g: |
化合物 | ChemComp-GOL: ChemComp-HOH: ChemComp-NAG: ChemComp-CL: |
由来 |
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キーワード | IMMUNE SYSTEM (免疫系) / IGG Fold / Antibody (抗体) / HIV-1 binding (ヒト免疫不全ウイルス) / Protein-Protein complex / IgG / Anti-HIV antibodies / gp120 (Gp120 (HIV)) |