EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Changes within the central stalk of E. coli FF ATP synthase observed after addition of ATP.
ジャーナル・号・ページ	Commun Biol, Vol. 6, Issue 1, Page 26, Year 2023
掲載日	2023年1月11日
著者	Meghna Sobti / Yi C Zeng / James L Walshe / Simon H J Brown / Robert Ishmukhametov / Alastair G Stewart /
PubMed 要旨	FF ATP synthase functions as a biological generator and makes a major contribution to cellular energy production. Proton flow generates rotation in the F motor that is transferred to the F motor to ...FF ATP synthase functions as a biological generator and makes a major contribution to cellular energy production. Proton flow generates rotation in the F motor that is transferred to the F motor to catalyze ATP production, with flexible F/F coupling required for efficient catalysis. FF ATP synthase can also operate in reverse, hydrolyzing ATP and pumping protons, and in bacteria this function can be regulated by an inhibitory ε subunit. Here we present cryo-EM data showing E. coli FF ATP synthase in different rotational and inhibited sub-states, observed following incubation with 10 mM MgATP. Our structures demonstrate how structural transitions within the inhibitory ε subunit induce torsional movement in the central stalk, thereby enabling its rotation within the F motor. This highlights the importance of the central rotor for flexible coupling of the F and F motors and provides further insight into the regulatory mechanism mediated by subunit ε.
リンク	Commun Biol / PubMed:36631659 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 7.8 Å
構造データ	EMDB-27296: E. coli ATP synthase imaged in 10mM MgATP State1 手法: EM (単粒子) / 解像度: 3.0 Å 27297 8dbp EMDB-27297, PDB-8dbp: E. coli ATP synthase imaged in 10mM MgATP State1 "half-up 手法: EM (単粒子) / 解像度: 3.6 Å 27298 8dbq EMDB-27298, PDB-8dbq: E. coli ATP synthase imaged in 10mM MgATP State1 "half-up" Fo classified 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-27299: E. coli ATP synthase imaged in 10mM MgATP State 1 "half-up" Fo refine 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-27300: E. coli ATP synthase imaged in 10mM MgATP State1 "down" 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-27301: E. coli ATP synthase imaged in 10mM MgATP State 1 "down" Fo classified 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-27302: E. coli ATP synthase imaged in 10mM MgATP State2 手法: EM (単粒子) / 解像度: 2.7 Å 27303 8dbr EMDB-27303, PDB-8dbr: E. coli ATP synthase imaged in 10mM MgATP State2 "half-up 手法: EM (単粒子) / 解像度: 3.2 Å 27304 8dbs EMDB-27304, PDB-8dbs: E. coli ATP synthase imaged in 10mM MgATP State2 "half-up" Fo classified 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-27305: E. coli ATP synthase imaged in 10mM MgATP State2 "half-up" Fo refine 手法: EM (単粒子) / 解像度: 7.0 Å 27306 8dbt EMDB-27306, PDB-8dbt: E. coli ATP synthase imaged in 10mM MgATP State2 "down 手法: EM (単粒子) / 解像度: 3.1 Å 27307 8dbu EMDB-27307, PDB-8dbu: E. coli ATP synthase imaged in 10mM MgATP State2 "down" Fo classified 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-27308: E. coli ATP synthase imaged in 10mM MgATP State2 "down" Fo refine 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-27309: E. coli ATP synthase imaged in 10mM MgATP State3 手法: EM (単粒子) / 解像度: 3.0 Å 27310 8dbv EMDB-27310, PDB-8dbv: E. coli ATP synthase imaged in 10mM MgATP State3 "down 手法: EM (単粒子) / 解像度: 3.7 Å 27311 8dbw EMDB-27311, PDB-8dbw: E. coli ATP synthase imaged in 10mM MgATP State3 "down" Fo classified 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-27312: E. coli ATP synthase imaged in 10mM MgATP State3 "down" Fo refine 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-27313: E. coli ATP synthase epsilon helix1 deletion mutant imaged in 10mM MgATP State1 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-27314: E. coli ATP synthase epsilon helix1 deletion mutant imaged in 10mM MgATP State2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-27315: E. coli ATP synthase epsilon helix1 deletion mutant imaged in 10mM MgATP State3 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Energy (エネルギー) / ATP hyrolysis / ATP synthesis (ATP合成酵素) / Motor (機関 (機械)) / cryoEM (低温電子顕微鏡法)