EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and gating mechanisms of human bestrophin anion channels.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 3836, Year 2022
掲載日	2022年7月4日
著者	Aaron P Owji / Jiali Wang / Alec Kittredge / Zada Clark / Yu Zhang / Wayne A Hendrickson / Tingting Yang /
PubMed 要旨	Bestrophin-1 (Best1) and bestrophin-2 (Best2) are two members of the bestrophin family of calcium (Ca)-activated chloride (Cl) channels with critical involvement in ocular physiology and direct ...Bestrophin-1 (Best1) and bestrophin-2 (Best2) are two members of the bestrophin family of calcium (Ca)-activated chloride (Cl) channels with critical involvement in ocular physiology and direct pathological relevance. Here, we report cryo-EM structures of wild-type human Best1 and Best2 in various states at up to 1.8 Å resolution. Ca-bound Best1 structures illustrate partially open conformations at the two Ca-dependent gates of the channels, in contrast to the fully open conformations observed in Ca-bound Best2, which is in accord with the significantly smaller currents conducted by Best1 in electrophysiological recordings. Comparison of the closed and open states reveals a C-terminal auto-inhibitory segment (AS), which constricts the channel concentrically by wrapping around the channel periphery in an inter-protomer manner and must be released to allow channel opening. Our results demonstrate that removing the AS from Best1 and Best2 results in truncation mutants with similar activities, while swapping the AS between Best1 and Best2 results in chimeric mutants with swapped activities, underlying a key role of the AS in determining paralog specificity among bestrophins.
リンク	Nat Commun / PubMed:35789156 / PubMed Central
手法	EM (単粒子)
解像度	1.78 - 3.11 Å
構造データ	27127 8d1e EMDB-27127, PDB-8d1e: hBest2 1uM Ca2+ (Ca2+-bound) closed state 手法: EM (単粒子) / 解像度: 1.78 Å 27128 8d1f EMDB-27128, PDB-8d1f: hBest2 5mM Ca2+ (Ca2+-bound) closed state 手法: EM (単粒子) / 解像度: 1.82 Å 27129 8d1g EMDB-27129, PDB-8d1g: hBest2 Ca2+-bound open state 手法: EM (単粒子) / 解像度: 2.07 Å 27130 8d1h EMDB-27130, PDB-8d1h: hBest2 Ca2+-unbound closed state 手法: EM (単粒子) / 解像度: 1.94 Å 27131 8d1i EMDB-27131, PDB-8d1i: hBest1 1uM Ca2+ (Ca2+-bound) closed state 手法: EM (単粒子) / 解像度: 1.82 Å 27132 8d1j EMDB-27132, PDB-8d1j: hBest1 5mM Ca2+ (Ca2+-bound) closed state 手法: EM (単粒子) / 解像度: 2.05 Å 27133 8d1k EMDB-27133, PDB-8d1k: hBest1 Ca2+-bound partially open neck state 手法: EM (単粒子) / 解像度: 2.28 Å 27134 8d1l EMDB-27134, PDB-8d1l: hBest1 Ca2+-bound partially open aperture state 手法: EM (単粒子) / 解像度: 2.12 Å 27135 8d1m EMDB-27135, PDB-8d1m: hBest1 Ca2+-unbound closed state 手法: EM (単粒子) / 解像度: 3.11 Å 27136 8d1n EMDB-27136, PDB-8d1n: bBest2_345 Ca2+-bound open state 手法: EM (単粒子) / 解像度: 1.93 Å 27137 8d1o EMDB-27137, PDB-8d1o: hBest1_345 Ca2+-bound open state 手法: EM (単粒子) / 解像度: 2.44 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-MC3: 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-DU0: 2-[2-[(1~{S},2~{S},4~{S},5'~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{R},16~{S})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-HOH*: WATER / 水
由来	homo sapiens (ヒト) bos taurus (ウシ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Ion channel (イオンチャネル) / chloride channel / anion channel (イオンチャネル) / pentamer