EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural analysis of receptor binding domain mutations in SARS-CoV-2 variants of concern that modulate ACE2 and antibody binding.
ジャーナル・号・ページ	Cell Rep, Vol. 37, Issue 12, Page 110156, Year 2021
掲載日	2021年12月21日
著者	Dhiraj Mannar / James W Saville / Xing Zhu / Shanti S Srivastava / Alison M Berezuk / Steven Zhou / Katharine S Tuttle / Andrew Kim / Wei Li / Dimiter S Dimitrov / Sriram Subramaniam /
PubMed 要旨	The recently emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Beta (B.1.351) and Gamma (P.1) variants of concern (VoCs) include a key mutation (N501Y) found in the Alpha (B.1.1.7) ...The recently emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Beta (B.1.351) and Gamma (P.1) variants of concern (VoCs) include a key mutation (N501Y) found in the Alpha (B.1.1.7) variant that enhances affinity of the spike protein for its receptor, angiotensin-converting enzyme 2 (ACE2). Additional mutations are found in these variants at residues 417 and 484 that appear to promote antibody evasion. In contrast, the Epsilon variants (B.1.427/429) lack the N501Y mutation yet exhibit antibody evasion. We have engineered spike proteins to express these receptor binding domain (RBD) VoC mutations either in isolation or in different combinations and analyze the effects using biochemical assays and cryoelectron microscopy (cryo-EM) structural analyses. Overall, our findings suggest that the emergence of new SARS-CoV-2 variant spikes can be rationalized as the result of mutations that confer increased ACE2 affinity, increased antibody evasion, or both, providing a framework to dissect the molecular factors that drive VoC evolution.
リンク	Cell Rep / PubMed:34914928 / PubMed Central
手法	EM (単粒子)
解像度	2.31 - 3.35 Å
構造データ	25503 7sxr EMDB-25503, PDB-7sxr: Cryo-EM structure of the SARS-CoV-2 D614G mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 3.0 Å 25504 7sxs EMDB-25504, PDB-7sxs: Cryo-EM structure of the SARS-CoV-2 D614G,L452R mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 2.64 Å 25505 7sxt EMDB-25505, PDB-7sxt: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 2.31 Å 25506 7sxu EMDB-25506, PDB-7sxu: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 3.0 Å 25507 7sxv EMDB-25507, PDB-7sxv: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K,K417N mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 2.79 Å 25508 7sxw EMDB-25508, PDB-7sxw: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K,K417T mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 2.85 Å 25509 7sxx EMDB-25509, PDB-7sxx: Cryo-EM structure of the SARS-CoV-2 D614G mutant spike protein ectodomain bound to human ACE2 ectodomain (global refinement) 手法: EM (単粒子) / 解像度: 2.66 Å 25510 7sxy EMDB-25510, PDB-7sxy: Cryo-EM structure of the SARS-CoV-2 D614G mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 2.79 Å 25511 7sxz EMDB-25511, PDB-7sxz: Cryo-EM structure of the SARS-CoV-2 D614G,L452R mutant spike protein ectodomain bound to human ACE2 ectodomain (global refinement) 手法: EM (単粒子) / 解像度: 2.61 Å 25512 7sy0 EMDB-25512, PDB-7sy0: Cryo-EM structure of the SARS-CoV-2 D614G,L452R mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.0 Å 25513 7sy1 EMDB-25513, PDB-7sy1: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y mutant spike protein ectodomain bound to human ACE2 ectodomain (global refinement) 手法: EM (単粒子) / 解像度: 2.83 Å 25514 7sy2 EMDB-25514, PDB-7sy2: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.11 Å 25515 7sy3 EMDB-25515, PDB-7sy3: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K mutant spike protein ectodomain bound to human ACE2 ectodomain (global refinement) 手法: EM (単粒子) / 解像度: 2.95 Å 25516 7sy4 EMDB-25516, PDB-7sy4: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.35 Å 25517 7sy5 EMDB-25517, PDB-7sy5: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K,K417N mutant spike protein ectodomain bound to human ACE2 ectodomain (global refinement) 手法: EM (単粒子) / 解像度: 2.59 Å 25518 7sy6 EMDB-25518, PDB-7sy6: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K,K417N mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 2.81 Å 25519 7sy7 EMDB-25519, PDB-7sy7: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K,K417T mutant spike protein ectodomain bound to human ACE2 ectodomain (global refinement) 手法: EM (単粒子) / 解像度: 2.81 Å 25520 7sy8 EMDB-25520, PDB-7sy8: Cryo-EM structure of the SARS-CoV-2 D614G,N501Y,E484K,K417T mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.14 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / SARS-CoV-2 (SARSコロナウイルス2) / glycoprotein (糖タンパク質) / fusion protein (融合タンパク質) / Viral Protein/Hydrolase (ウイルス性) / ACE2 (アンジオテンシン変換酵素2) / Viral Protein-Hydrolase complex (ウイルス性) / VIRAL PROTEIN/RECEPTOR (ウイルス性) / VIRAL PROTEIN-RECEPTOR complex (ウイルス性)