EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Negative charge in the RACK1 loop broadens the translational capacity of the human ribosome.
ジャーナル・号・ページ	Cell Rep, Vol. 36, Issue 10, Page 109663, Year 2021
掲載日	2021年9月7日
著者	Madeline G Rollins / Manidip Shasmal / Nathan Meade / Helen Astar / Peter S Shen / Derek Walsh /
PubMed 要旨	Although the roles of initiation factors, RNA binding proteins, and RNA elements in regulating translation are well defined, how the ribosome functionally diversifies remains poorly understood. In ...Although the roles of initiation factors, RNA binding proteins, and RNA elements in regulating translation are well defined, how the ribosome functionally diversifies remains poorly understood. In their human hosts, poxviruses phosphorylate serine 278 (S) at the tip of a loop domain in the small subunit ribosomal protein RACK1, thereby mimicking negatively charged residues in the RACK1 loops of dicot plants and protists to stimulate translation of transcripts with 5' poly(A) leaders. However, how a negatively charged RACK1 loop affects ribosome structure and its broader translational output is not known. Here, we show that although ribotoxin-induced stress signaling and stalling on poly(A) sequences are unaffected, negative charge in the RACK1 loop alters the swivel motion of the 40S head domain in a manner similar to several internal ribosome entry sites (IRESs), confers resistance to various protein synthesis inhibitors, and broadly supports noncanonical modes of translation.
リンク	Cell Rep / PubMed:34496247 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 5.2 Å
構造データ	EMDB-24541: RACK1-S278E 80S rotated state, consensus reconstruction 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-24542: RACK1-S278E, 80S non-rotated state, consensus reconstruction 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-24544: RACK1-S278E, reconstruction from 40S particles (filtered according to local resolution) 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-24545: RACK1-WT, 80S rotated state, consensus reconstruction 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-24548: RACK1-WT, 80S nonrotated consensus reconstruction 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-24549: RACK1-S278E, 60S masked from 80S rotated state, locally refined and filtered 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-24552: RACK1-S278E, 40S body masked from 80S rotated state, locally refined and filtered 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-24553: RACK1-S278E, 40S head masked from 80S rotated state, locally refined and filtered 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-24554: RACK1-WT, 60S masked from 80S rotated state, locally refined and filtered 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-24556: RACK1-WT, 40S body masked from 80S rotated state, locally refined and filtered 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-24557: RACK1-WT, 40S head masked from 80S rotated state, locally refined and filtered 手法: EM (単粒子) / 解像度: 4.5 Å
由来	Homo sapiens (ヒト)