EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 7, Page 564-572, Year 2021
掲載日	2021年7月8日
著者	Kirill D Nadezhdin / Arthur Neuberger / Yuri A Trofimov / Nikolay A Krylov / Viktor Sinica / Nikita Kupko / Viktorie Vlachova / Eleonora Zakharian / Roman G Efremov / Alexander I Sobolevsky /
PubMed 要旨	Numerous physiological functions rely on distinguishing temperature through temperature-sensitive transient receptor potential channels (thermo-TRPs). Although the function of thermo-TRPs has been ...Numerous physiological functions rely on distinguishing temperature through temperature-sensitive transient receptor potential channels (thermo-TRPs). Although the function of thermo-TRPs has been studied extensively, structural determination of their heat- and cold-activated states has remained a challenge. Here, we present cryo-EM structures of the nanodisc-reconstituted wild-type mouse TRPV3 in three distinct conformations: closed, heat-activated sensitized and open states. The heat-induced transformations of TRPV3 are accompanied by changes in the secondary structure of the S2-S3 linker and the N and C termini and represent a conformational wave that links these parts of the protein to a lipid occupying the vanilloid binding site. State-dependent differences in the behavior of bound lipids suggest their active role in thermo-TRP temperature-dependent gating. Our structural data, supported by physiological recordings and molecular dynamics simulations, provide an insight for understanding the molecular mechanism of temperature sensing.
リンク	Nat Struct Mol Biol / PubMed:34239124 / PubMed Central
手法	EM (単粒子)
解像度	1.98 - 3.86 Å
構造データ	23853 7mij EMDB-23853, PDB-7mij: Mouse TRPV3 in MSP2N2 nanodiscs, closed state at 4 degrees Celsius 手法: EM (単粒子) / 解像度: 1.98 Å 23854 7mik EMDB-23854, PDB-7mik: Mouse TRPV3 in MSP2N2 nanodiscs, closed state at 42 degrees Celsius 手法: EM (単粒子) / 解像度: 3.12 Å 23855 7mil EMDB-23855, PDB-7mil: Mouse TRPV3 in MSP2N2 nanodiscs, sensitized state at 42 degrees Celsius 手法: EM (単粒子) / 解像度: 3.86 Å 23856 7mim EMDB-23856, PDB-7mim: Mouse TRPV3 in cNW11 nanodiscs, closed state at 4 degrees Celsius 手法: EM (単粒子) / 解像度: 3.42 Å 23857 7min EMDB-23857, PDB-7min: Mouse TRPV3 in cNW11 nanodiscs, closed state at 42 degrees Celsius 手法: EM (単粒子) / 解像度: 3.09 Å 23858 7mio EMDB-23858, PDB-7mio: Mouse TRPV3 in cNW11 nanodiscs, open state at 42 degrees Celsius 手法: EM (単粒子) / 解像度: 3.48 Å
化合物	ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM / POホスファチジルコリン ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH*: WATER / 水
由来	mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Transient Receptor Potential V Family Member 3 / TRP channel (TRPチャネル) / TRPV3 / closed state at 4 degrees Celsius / MSP2N2 / closed state at 42 degrees Celsius / sensitized state at 42 degrees Celsius / cNW11 / open state at 42 degrees Celsius