EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for target site selection in RNA-guided DNA transposition systems.
ジャーナル・号・ページ	Science, Vol. 373, Issue 6556, Page 768-774, Year 2021
掲載日	2021年8月13日
著者	Jung-Un Park / Amy Wei-Lun Tsai / Eshan Mehrotra / Michael T Petassi / Shan-Chi Hsieh / Ailong Ke / Joseph E Peters / Elizabeth H Kellogg /
PubMed 要旨	CRISPR-associated transposition systems allow guide RNA-directed integration of a single DNA cargo in one orientation at a fixed distance from a programmable target sequence. We used cryo-electron ...CRISPR-associated transposition systems allow guide RNA-directed integration of a single DNA cargo in one orientation at a fixed distance from a programmable target sequence. We used cryo-electron microscopy (cryo-EM) to define the mechanism that underlies this process by characterizing the transposition regulator, TnsC, from a type V-K CRISPR-transposase system. In this scenario, polymerization of adenosine triphosphate-bound TnsC helical filaments could explain how polarity information is passed to the transposase. TniQ caps the TnsC filament, representing a universal mechanism for target information transfer in Tn7/Tn7-like elements. Transposase-driven disassembly establishes delivery of the element only to unused protospacers. Finally, TnsC transitions to define the fixed point of insertion, as revealed by structures with the transition state mimic ADP•AlF These mechanistic findings provide the underpinnings for engineering CRISPR-associated transposition systems for research and therapeutic applications.
リンク	Science / PubMed:34385391 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.2 - 4.2 Å
構造データ	23720 7m99 EMDB-23720, PDB-7m99: ATPgS bound TnsC filament from ShCAST system 手法: EM (らせん対称) / 解像度: 3.2 Å 23721 7m9a EMDB-23721, PDB-7m9a: ADP-AlF3 bound TnsC structure from ShCAST system 手法: EM (単粒子) / 解像度: 3.9 Å 23722 7m9b EMDB-23722, PDB-7m9b: ADP-AlF3 bound TnsC structure in closed form 手法: EM (単粒子) / 解像度: 3.8 Å 23723 7m9c EMDB-23723, PDB-7m9c: ADP-AlF3 bound TnsC structure in open form 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-23724: ATP bound TnsC filament structure from ShCAST system 手法: EM (らせん対称) / 解像度: 3.7 Å EMDB-23725: Head-to-head filament of TnsC from ShCAST system 手法: EM (単粒子) / 解像度: 4.2 Å 23726 7n6i EMDB-23726, PDB-7n6i: ATP-bound TnsC-TniQ complex from ShCAST system 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM / アデノシン三リン酸
由来	scytonema hofmannii (バクテリア) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN/DNA / CRISPR (CRISPR) / Transposition / AAA+ ATPase / Tn7 / DNA BINDING PROTEIN-DNA complex / DNA BINDING PROTEIN (DNA結合タンパク質) / Transposase