EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.
ジャーナル・号・ページ	Cell, Vol. 183, Issue 4, Page 1024-1042.e21, Year 2020
掲載日	2020年11月12日
著者	Luca Piccoli / Young-Jun Park / M Alejandra Tortorici / Nadine Czudnochowski / Alexandra C Walls / Martina Beltramello / Chiara Silacci-Fregni / Dora Pinto / Laura E Rosen / John E Bowen / Oliver J Acton / Stefano Jaconi / Barbara Guarino / Andrea Minola / Fabrizia Zatta / Nicole Sprugasci / Jessica Bassi / Alessia Peter / Anna De Marco / Jay C Nix / Federico Mele / Sandra Jovic / Blanca Fernandez Rodriguez / Sneha V Gupta / Feng Jin / Giovanni Piumatti / Giorgia Lo Presti / Alessandra Franzetti Pellanda / Maira Biggiogero / Maciej Tarkowski / Matteo S Pizzuto / Elisabetta Cameroni / Colin Havenar-Daughton / Megan Smithey / David Hong / Valentino Lepori / Emiliano Albanese / Alessandro Ceschi / Enos Bernasconi / Luigia Elzi / Paolo Ferrari / Christian Garzoni / Agostino Riva / Gyorgy Snell / Federica Sallusto / Katja Fink / Herbert W Virgin / Antonio Lanzavecchia / Davide Corti / David Veesler /
PubMed 要旨	Analysis of the specificity and kinetics of neutralizing antibodies (nAbs) elicited by SARS-CoV-2 infection is crucial for understanding immune protection and identifying targets for vaccine design. ...Analysis of the specificity and kinetics of neutralizing antibodies (nAbs) elicited by SARS-CoV-2 infection is crucial for understanding immune protection and identifying targets for vaccine design. In a cohort of 647 SARS-CoV-2-infected subjects, we found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores. The receptor-binding domain (RBD) is immunodominant and the target of 90% of the neutralizing activity present in SARS-CoV-2 immune sera. Whereas overall RBD-specific serum IgG titers waned with a half-life of 49 days, nAb titers and avidity increased over time for some individuals, consistent with affinity maturation. We structurally defined an RBD antigenic map and serologically quantified serum Abs specific for distinct RBD epitopes leading to the identification of two major receptor-binding motif antigenic sites. Our results explain the immunodominance of the receptor-binding motif and will guide the design of COVID-19 vaccines and therapeutics.
リンク	Cell / PubMed:32991844 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.043 - 8.5 Å
構造データ	22491 7jv2 EMDB-22491, PDB-7jv2: SARS-CoV-2 spike in complex with the S2H13 neutralizing antibody Fab fragment (local refinement of the receptor-binding motif and Fab variable domains) 手法: EM (単粒子) / 解像度: 3.5 Å 22492 7jv4 EMDB-22492, PDB-7jv4: SARS-CoV-2 spike in complex with the S2H13 neutralizing antibody (one RBD open) 手法: EM (単粒子) / 解像度: 3.4 Å 22494 7jv6 EMDB-22494, PDB-7jv6: SARS-CoV-2 spike in complex with the S2H13 neutralizing antibody (closed conformation) 手法: EM (単粒子) / 解像度: 3.0 Å 22497 7jva EMDB-22497, PDB-7jva: SARS-CoV-2 spike in complex with the S2A4 neutralizing antibody Fab fragment (local refinement of the receptor-binding domain and Fab variable domains) 手法: EM (単粒子) / 解像度: 3.6 Å 22506 7jvc EMDB-22506, PDB-7jvc: SARS-CoV-2 spike in complex with the S2A4 neutralizing antibody Fab fragment 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-22507: SARS-CoV-2 spike in complex with the S2H14 neutralizing antibody Fab fragment (two receptor-binding domains open) 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-22508: SARS-CoV-2 spike in complex with the S2H14 neutralizing antibody Fab fragment (three receptor-binding domains open) 手法: EM (単粒子) / 解像度: 8.5 Å 22512 7jw0 EMDB-22512, PDB-7jw0: SARS-CoV-2 spike in complex with the S304 neutralizing antibody Fab fragment 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-22516: SARS-CoV-2 spike in complex with the S2X35 neutralizing antibody Fab fragment 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-22517: SARS-CoV-2 spike in complex with the S2X35 neutralizing antibody Fab fragment (local refinement of the receptor-binding motif and Fab variable domains) 手法: EM (単粒子) / 解像度: 5.0 Å PDB-7jx3: Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology 手法: X-RAY DIFFRACTION / 解像度: 2.65 Å PDB-7jxc: Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology 手法: X-RAY DIFFRACTION / 解像度: 2.47 Å PDB-7jxd: Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-7jxe: Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology 手法: X-RAY DIFFRACTION / 解像度: 2.043 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-HOH: WATER / 水 ChemComp-2PE: NONAETHYLENE GLYCOL / 3,6,9,12,15,18,21,24-オクタオキサヘキサコサン-1,26-ジオ-ル / 沈殿剤*YM / ポリエチレングリコール
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / SARS-CoV-2 (SARSコロナウイルス2) / COVID-19 (新型コロナウイルス感染症 (2019年)) / spike glycoprotein (スパイクタンパク質) / fusion protein (融合タンパク質) / neutralizing antibodies (中和抗体) / Structural Genomics (構造ゲノミクス) / Seattle Structural Genomics Center for Infectious Disease / SSGCID / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) / neutralizing monoclonal antibody / IMMUNE SYSTEM (免疫系) / neutralizing antibody (中和抗体) / sarbecovirus (SARS関連コロナウイルス)