EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structures of natively assembled clathrin-coated vesicles.
ジャーナル・号・ページ	Sci Adv, Vol. 6, Issue 30, Page eaba8397, Year 2020
掲載日	2020年7月22日
著者	Mohammadreza Paraan / Joshua Mendez / Savanna Sharum / Danielle Kurtin / Huan He / Scott M Stagg /
PubMed 要旨	Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy ...Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.
リンク	Sci Adv / PubMed:32743076 / PubMed Central
手法	EM (単粒子)
解像度	6.3 - 17.9 Å
構造データ	EMDB-21608: Mini-coat geometry for a clathrin coated vesicle: clathrin-focused 手法: EM (単粒子) / 解像度: 8.5 Å EMDB-21609: Mini-coat geometry for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-21610: Basket geometry for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 15.6 Å 21611 6wcj EMDB-21611: Asymmetric vertex of the clathrin minicoat cage, subparticle refinement of the clathrin layer PDB-6wcj: Asymmetric vertex of the clathrin minicoat cage 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-21612: Football geometry for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 15.1 Å EMDB-21613: Tennis geometry for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 17.2 Å EMDB-21614: Barrel geometry for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 17.9 Å EMDB-21615: Pentagonal face of the mini-coat cage for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 8.3 Å EMDB-21616: Hexagonal face of the mini-coat cage for a clathrin coated vesicle 手法: EM (単粒子) / 解像度: 9.1 Å
由来	bos taurus (ウシ) bovine (ウシ)
キーワード	ENDOCYTOSIS (エンドサイトーシス) / Clathrin coated vesicle / clathrin heavy chain (クラスリン) / clathrin light chain (クラスリン) / clathrin cage