EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ribosome Dimerization Protects the Small Subunit.
ジャーナル・号・ページ	J Bacteriol, Vol. 202, Issue 10, Year 2020
掲載日	2020年4月27日
著者	Heather A Feaga / Mykhailo Kopylov / Jenny Kim Kim / Marko Jovanovic / Jonathan Dworkin /
PubMed 要旨	When nutrients become scarce, bacteria can enter an extended state of quiescence. A major challenge of this state is how to preserve ribosomes for the return to favorable conditions. Here, we show ...When nutrients become scarce, bacteria can enter an extended state of quiescence. A major challenge of this state is how to preserve ribosomes for the return to favorable conditions. Here, we show that the ribosome dimerization protein hibernation-promoting factor (HPF) functions to protect essential ribosomal proteins. Ribosomes isolated from strains lacking HPF (Δ) or encoding a mutant allele of HPF that binds the ribosome but does not mediate dimerization were substantially depleted of the small subunit proteins S2 and S3. Strikingly, these proteins are located directly at the ribosome dimer interface. We used single-particle cryo-electron microscopy (cryo-EM) to further characterize these ribosomes and observed that a high percentage of ribosomes were missing S2, S3, or both. These data support a model in which the ribosome dimerization activity of HPF evolved to protect labile proteins that are essential for ribosome function. HPF is almost universally conserved in bacteria, and HPF deletions in diverse species exhibit decreased viability during starvation. Our data provide mechanistic insight into this phenotype and establish a mechanism for how HPF protects ribosomes during quiescence. The formation of ribosome dimers during periods of dormancy is widespread among bacteria. Dimerization is typically mediated by a single protein, hibernation-promoting factor (HPF). Bacteria lacking HPF exhibit strong defects in viability and pathogenesis and, in some species, extreme loss of rRNA. The mechanistic basis of these phenotypes has not been determined. Here, we report that HPF from the Gram-positive bacterium preserves ribosomes by preventing the loss of essential ribosomal proteins at the dimer interface. This protection may explain phenotypes associated with the loss of HPF, since ribosome protection would aid survival during nutrient limitation and impart a strong selective advantage when the bacterial cell rapidly reinitiates growth in the presence of sufficient nutrients.
リンク	J Bacteriol / PubMed:32123037 / PubMed Central
手法	EM (単粒子)
解像度	4.3 - 4.9 Å
構造データ	EMDB-21467: Consensus map of a delta hpf 70S ribosome from Bacillus subtilis subsp. subtilis str. 168 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-21478: Class1 from classification of Bacillus subtilus delta HPF ribosomes 手法: EM (単粒子) / 解像度: 4.31 Å EMDB-21479: Class 2 from 3D classification of Bacillus subtilus delta HPF ribosomes 手法: EM (単粒子) / 解像度: 4.31 Å EMDB-21480: Class 3 from 3D classification of Bacillus subtilus delta HPF ribosomes 手法: EM (単粒子) / 解像度: 4.9 Å
由来	Bacillus subtilis subsp. subtilis str. 168 (枯草菌)