EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 116, Issue 49, Page 24786-24795, Year 2019
掲載日	2019年12月3日
著者	Carmen Butan / Maria Lara-Tejero / Wenwei Li / Jun Liu / Jorge E Galán /
PubMed 要旨	Type III protein secretion systems are essential virulence factors for many important pathogenic bacteria. The entire protein secretion machine is composed of several substructures that organize into ...Type III protein secretion systems are essential virulence factors for many important pathogenic bacteria. The entire protein secretion machine is composed of several substructures that organize into a holostructure or injectisome. The core component of the injectisome is the needle complex, which houses the export apparatus that serves as a gate for the passage of the secreted proteins through the bacterial inner membrane. Here, we describe a high-resolution structure of the export apparatus of the type III secretion system in association with the needle complex and the underlying bacterial membrane, both in isolation and in situ. We show the precise location of the core export apparatus components within the injectisome and bacterial envelope and demonstrate that their deployment results in major membrane remodeling and thinning, which may be central for the protein translocation process. We also show that InvA, a critical export apparatus component, forms a multiring cytoplasmic conduit that provides a pathway for the type III secretion substrates to reach the entrance of the export gate. Combined with structure-guided mutagenesis, our studies provide major insight into potential mechanisms of protein translocation and injectisome assembly.
リンク	Proc Natl Acad Sci U S A / PubMed:31744874 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	3.3 - 17.0 Å
構造データ	EMDB-20830: Cryo-EM reconstruction of the inner membrane rings containing the core components of the export apparatus and associated membranes of the needle complex from Salmonella typhimurium type III secretion system. 手法: EM (単粒子) / 解像度: 3.93 Å EMDB-20831: Cryo-EM reconstruction shows that the needle complex's inner rings from Salmonella assemble with 23-fold symmetry in the absence of the export apparatus. 手法: EM (単粒子) / 解像度: 5.3 Å 20832 6uot EMDB-20832, PDB-6uot: Cryo-EM structure of the PrgHK periplasmic ring from the Salmonella SPI-1 type III secretion needle complex solved at 3.3 angstrom resolution 手法: EM (単粒子) / 解像度: 3.3 Å 20833 6uov EMDB-20833, PDB-6uov: Cryo-EM reconstruction of the PrgHK periplasmic ring from Salmonella's needle complex assembled in the absence of the export apparatus 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-20838: In situ structure of the export apparatus in Salmonella T3SS machine 手法: EM (サブトモグラム平均) / 解像度: 17.0 Å
由来	salmonella enterica subsp. enterica serovar typhimurium (サルモネラ菌) Salmonella enterica subsp. enterica serovar Abony (サルモネラ菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / BACTERIAL NANOMACHINE / TYPE III SECRETION SYSTEM