EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the transition of Clostridioides difficile binary toxin from prepore to pore.
ジャーナル・号・ページ	Nat Microbiol, Vol. 5, Issue 1, Page 102-107, Year 2020
掲載日	2019年11月11日
著者	David M Anderson / Michael J Sheedlo / Jaime L Jensen / D Borden Lacy /
PubMed 要旨	Clostridioides (formerly Clostridium) difficile is a Gram-positive, spore-forming anaerobe and a leading cause of hospital-acquired infection and gastroenteritis-associated death in US hospitals. The ...Clostridioides (formerly Clostridium) difficile is a Gram-positive, spore-forming anaerobe and a leading cause of hospital-acquired infection and gastroenteritis-associated death in US hospitals. The disease state is usually preceded by disruption of the host microbiome in response to antibiotic treatment and is characterized by mild to severe diarrhoea. C. difficile infection is dependent on the secretion of one or more AB-type toxins: toxin A (TcdA), toxin B (TcdB) and the C. difficile transferase toxin (CDT). Whereas TcdA and TcdB are considered the primary virulence factors, recent studies suggest that CDT increases the severity of C. difficile infection in some of the most problematic clinical strains. To better understand how CDT functions, we used cryo-electron microscopy to define the structure of CDTb, the cell-binding component of CDT. We obtained structures of several oligomeric forms that highlight the conformational changes that enable conversion from a prepore to a β-barrel pore. The structural analysis also reveals a glycan-binding domain and residues involved in binding the host-cell receptor, lipolysis-stimulated lipoprotein receptor. Together, these results provide a framework to understand how CDT functions at the host cell interface.
リンク	Nat Microbiol / PubMed:31712627 / PubMed Central
手法	EM (単粒子)
解像度	3.7 - 6.3 Å
構造データ	0608 6o2m EMDB-0608: Reconstruction of CDTb Double Heptamer Long Form using C7 Symmetry PDB-6o2m: CDTb Double Heptamer Long Form Modeled from Cryo-EM Map Reconstructed using C7 Symmetry 手法: EM (単粒子) / 解像度: 6.3 Å 0609 6o2n EMDB-0609: Reconstruction of CDTb Double Heptamer Short Form using C7 Symmetry PDB-6o2n: CDTb Double Heptamer Short Form Modeled from Cryo-EM Map Reconstructed using C7 Symmetry 手法: EM (単粒子) / 解像度: 3.7 Å 0610 6o2o EMDB-0610: Reconstruction of CDTb Double Heptamer Short Form using C1 Symmetry PDB-6o2o: CDTb Double Heptamer Short Form Modeled from Cryo-EM Map Reconstructed using C1 Symmetry 手法: EM (単粒子) / 解像度: 4.53 Å 20102 6okr EMDB-20102, PDB-6okr: CDTb Pre-Insertion form Modeled from Cryo-EM Map Reconstructed using C7 Symmetry 手法: EM (単粒子) / 解像度: 4.2 Å 20103 6oks EMDB-20103, PDB-6oks: CDTb Double Heptamer Long Form Mask 1 Modeled from Cryo-EM Map Reconstructed using C7 Symmetry 手法: EM (単粒子) / 解像度: 4.2 Å 20104 6okt EMDB-20104, PDB-6okt: CDTb Double Heptamer Long Form Mask 1 Modeled from Cryo-EM Map Reconstructed using C7 Symmetry 手法: EM (単粒子) / 解像度: 4.2 Å 20105 6oku EMDB-20105, PDB-6oku: CDTb Double Heptamer Long Form Mask 3 Modeled from Cryo-EM Map Reconstructed using C7 Symmetry 手法: EM (単粒子) / 解像度: 3.8 Å
由来	clostridioides difficile (クロストリディオイデス・ディフィシル)
キーワード	TRANSFERASE (転移酵素) / CDTb / Clostridium (クロストリジウム属) / Toxin (毒素) / Binary / difficil / difficile