EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	SCAF1 drives the compositional diversity of mammalian respirasomes.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年4月4日
著者	Irene Vercellino / Leonid A Sazanov /
PubMed 要旨	Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies ...Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies have shown that different tissues/organs are equipped with specific sets of supercomplexes, depending on their metabolic needs, the notion that supercomplexes have a role in the regulation of metabolism has been challenged. However, irrespective of the mechanistic conclusions, the composition of various high molecular weight supercomplexes remains uncertain. Here, using cryogenic electron microscopy, we demonstrate that mammalian (mouse) tissues contain three defined types of 'respirasome', supercomplexes made of CI, CIII and CIV. The stoichiometry and position of CIV differs in the three respirasomes, of which only one contains the supercomplex-associated factor SCAF1, whose involvement in respirasome formation has long been contended. Our structures confirm that the 'canonical' respirasome (the C-respirasome, CICIIICIV) does not contain SCAF1, which is instead associated to a different respirasome (the CS-respirasome), containing a second copy of CIV. We also identify an alternative respirasome (A-respirasome), with CIV bound to the 'back' of CI, instead of the 'toe'. This structural characterization of mouse mitochondrial supercomplexes allows us to hypothesize a mechanistic basis for their specific role in different metabolic conditions.
リンク	Nat Struct Mol Biol / PubMed:38575788
手法	EM (単粒子)
解像度	2.7 - 3.8 Å
構造データ	17989 8pw5 EMDB-17989, PDB-8pw5: CS respirasome from murine liver 手法: EM (単粒子) / 解像度: 3.6 Å 17990 8pw6 EMDB-17990, PDB-8pw6: C respirasome from murine liver 手法: EM (単粒子) / 解像度: 3.3 Å 17991 8pw7 EMDB-17991, PDB-8pw7: A respirasome from murine liver 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18011: focused map of complex I membrane arm from A respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-18012: focused map of complex I peripheral arm from A respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-18013: focused map of complex III from A prespirasome, murine liver 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-18014: focused map of CIV from A respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18015: consensus refinement of A respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18017: focused map of complex I peripheral arm from C respirasome, murine liver 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-18018: focused map of complex I membrane arm from C respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-18019: focused map of complex III from C respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-18020: focused map of complex IV from C respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-18021: global refinement of C respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-18022: focused map of complex I peripheral arm from SC respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-18023: focused map of complex I membrane arm from SC respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-18024: focused map of the canonical CIV from SC respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-18025: focused map of complex III from SC respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-18026: focused map of the SCAF1-containing complex IV from SC respirasome, murine livers 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18027: global refinement of SC respirasome, murine liver 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-19085: global map of murine liver closed complex I 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-19086: focused map of murine liver complex I peripheral arm in the closed conformation 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-19087: focused map of murine liver complex I membrane arm in the closed conformation 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-19088: global refinement of murine liver complex I in the open conformation 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-19089: focused map of the peripheral arm of complex I from murine liver in the open conformation 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-19090: focused map of the membrane arm of complex I from murine liver in the open conformation 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-19091: global refinement of murine brain complex I in the closed conformation 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-19092: focused map of the peripheral arm of complex I from murine brain in the closed conformation 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-19093: focused map of the membrane arm of murine brain complex I in the closed conformation 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-19105: global refinement of complex I from murine brain in the open conformation 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-19106: focused map of the peripheral arm of murine brain complex I in the open conformation 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-19107: focused map of the membrane arm of murine brain complex I in the open conformation 手法: EM (単粒子) / 解像度: 3.1 Å 19145 8rgp EMDB-19145, PDB-8rgp: Closed Complex I from murine brain 手法: EM (単粒子) / 解像度: 3.0 Å 19146 8rgq EMDB-19146, PDB-8rgq: Open Complex I from murine liver 手法: EM (単粒子) / 解像度: 3.0 Å 19147 8rgr EMDB-19147, PDB-8rgr: Closed Complex I from murine liver 手法: EM (単粒子) / 解像度: 2.9 Å 19148 8rgt EMDB-19148, PDB-8rgt: Open Complex I from murine brain 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-HEC: HEME C / Heme C ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-CU: COPPER (II) ION / 銅 ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HEA: HEME-A / Heme A ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-ZN: Unknown entry ChemComp-TGL: TRISTEAROYLGLYCEROL / トリステアリン / ステアリン ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN / フラビンモノヌクレオチド ChemComp-K: Unknown entry / カリウムカチオン ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH / ニコチンアミドアデニンジヌクレオチドリン酸 ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-DGT: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / dGTP / デオキシグアノシン三リン酸 ChemComp-UQ*: Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer
由来	mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Respiratory chain super complex / mammalian mitochondria / ELECTRON TRANSPORT (電子伝達系) / respiratory chain complex (電子伝達系)