EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 23, Page 5054-5067.e16, Year 2023
掲載日	2023年11月9日
著者	Kashish Singh / Georg Bunzel / Benjamin Graf / Ka Man Yip / Meina Neumann-Schaal / Holger Stark / Ashwin Chari /
PubMed 要旨	Fatty acids (FAs) play a central metabolic role in living cells as constituents of membranes, cellular energy reserves, and second messenger precursors. A 2.6 MDa FA synthase (FAS), where the ...Fatty acids (FAs) play a central metabolic role in living cells as constituents of membranes, cellular energy reserves, and second messenger precursors. A 2.6 MDa FA synthase (FAS), where the enzymatic reactions and structures are known, is responsible for FA biosynthesis in yeast. Essential in the yeast FAS catalytic cycle is the acyl carrier protein (ACP) that actively shuttles substrates, biosynthetic intermediates, and products from one active site to another. We resolve the S. cerevisiae FAS structure at 1.9 Å, elucidating cofactors and water networks involved in their recognition. Structural snapshots of ACP domains bound to various enzymatic domains allow the reconstruction of a full yeast FA biosynthesis cycle. The structural information suggests that each FAS functional unit could accommodate exogenous proteins to incorporate various enzymatic activities, and we show proof-of-concept experiments where ectopic proteins are used to modulate FAS product profiles.
リンク	Cell / PubMed:37949058
手法	EM (単粒子)
解像度	1.9 - 4.0 Å
構造データ	17839 8prv EMDB-17839, PDB-8prv: Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with ACP at the ketosreductase domain (FASamn sample) 手法: EM (単粒子) / 解像度: 2.9 Å 17840 8prw EMDB-17840, PDB-8prw: Cryo-EM structure of the yeast fatty acid synthase at 1.9 angstrom resolution 手法: EM (単粒子) / 解像度: 1.9 Å 17842 8ps1 EMDB-17842, PDB-8ps1: Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with ACP at the ketosynthase domain (FASamn sample) 手法: EM (単粒子) / 解像度: 2.8 Å 17843 8ps2 EMDB-17843, PDB-8ps2: Asymmetric unit of the yeast fatty acid synthase with ACP at the enoyl reductase domain (FASam sample) 手法: EM (単粒子) / 解像度: 2.9 Å 17846 8ps8 EMDB-17846, PDB-8ps8: Asymmetric unit of the yeast fatty acid synthase in the semi non-rotated state with ACP at the enoyl reductase domain (FASam sample) 手法: EM (単粒子) / 解像度: 4.0 Å 17847 8ps9 EMDB-17847, PDB-8ps9: Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with ACP at the ketosynthase domain (FASam sample) 手法: EM (単粒子) / 解像度: 2.9 Å 17848 8psa EMDB-17848, PDB-8psa: Asymmetric unit of the yeast fatty acid synthase in the semi non-rotated state with ACP at the ketosynthase domain (FASam sample) 手法: EM (単粒子) / 解像度: 3.6 Å 17851 8psf EMDB-17851, PDB-8psf: Asymmetric unit of the yeast fatty acid synthase in non-rotated state with ACP at the acetyl transferase domain (FASx sample) 手法: EM (単粒子) / 解像度: 2.8 Å 17852 8psg EMDB-17852, PDB-8psg: Asymmetric unit of the yeast fatty acid synthase in the semi non-rotated state with ACP at the acetyl transferase domain (FASx sample) 手法: EM (単粒子) / 解像度: 3.7 Å 17853 8psj EMDB-17853, PDB-8psj: Asymmetric unit of the yeast fatty acid synthase in the semi rotated state with ACP at the acetyl transferase domain (FASx sample) 手法: EM (単粒子) / 解像度: 3.4 Å 17854 8psk EMDB-17854, PDB-8psk: Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with ACP at the ketosynthase domain (FASx sample) 手法: EM (単粒子) / 解像度: 2.8 Å 17855 8psl EMDB-17855, PDB-8psl: Asymmetric unit of the yeast fatty acid synthase in the semi non-rotated state with ACP at the ketosynthase domain (FASx sample) 手法: EM (単粒子) / 解像度: 3.7 Å 17856 8psm EMDB-17856, PDB-8psm: Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with ACP at the malonyl/palmitoyl transferase domain (FASx sample) 手法: EM (単粒子) / 解像度: 3.1 Å 17859 8psp EMDB-17859, PDB-8psp: Asymmetric unit of the yeast fatty acid synthase in rotated state with ACP at the acetyl transferase domain (FASx sample) 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-NAP: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / ニコチンアミドアデニンジヌクレオチドリン酸 ChemComp-A5S: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] 3-oxidanylidenebutanethioate ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN / フラビンモノヌクレオチド ChemComp-COA: COENZYME A / CoA / 補酵素A ChemComp-FNR: 1-DEOXY-1-(7,8-DIMETHYL-2,4-DIOXO-3,4-DIHYDRO-2H-BENZO[G]PTERIDIN-1-ID-10(5H)-YL)-5-O-PHOSPHONATO-D-RIBITOL / 還元型フラビンモノヌクレオチド ChemComp-HOH: WATER / 水 ChemComp-A3Z: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (~{E})-but-2-enethioate ChemComp-MLC: MALONYL-COENZYME A / マロニルCoA / マロニルCoA ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸 / Phosphopantetheine
由来	saccharomyces cerevisiae (パン酵母)
キーワード	CYTOSOLIC PROTEIN (細胞質基質) / Fatty acid synthase (脂肪酸合成酵素) / acyl carrier protein / FAS / ACP