EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Super-resolution fluorescence imaging of cryosamples does not limit achievable resolution in cryoEM.
ジャーナル・号・ページ	J Struct Biol, Vol. 215, Issue 4, Page 108040, Year 2023
掲載日	2023年10月31日
著者	Mart G F Last / Willem E M Noteborn / Lenard M Voortman / Thomas H Sharp /
PubMed 要旨	Correlated super-resolution cryo-fluorescence and cryo-electron microscopy (cryoEM) has been gaining popularity as a method to investigate biological samples with high resolution and specificity. A ...Correlated super-resolution cryo-fluorescence and cryo-electron microscopy (cryoEM) has been gaining popularity as a method to investigate biological samples with high resolution and specificity. A concern in this combined method (called SR-cryoCLEM), however, is whether and how fluorescence imaging prior to cryoEM acquisition is detrimental to sample integrity. In this report, we investigated the effect of high-dose laser light (405, 488, and 561 nm) irradiation on apoferritin samples prepared for cryoEM with excitation wavelengths commonly used in fluorescence microscopy, and compared these samples to controls that were kept in the dark. We found that laser illumination, of equal duration and intensity as used in cryo-single molecule localization microscopy (cryoSMLM) and in the presence of high concentrations of fluorescent protein, did not affect the achievable resolution in cryoEM, with final reconstructions reaching resolutions of ∼ 1.8 Å regardless of the laser illumination. The finding that super-resolution fluorescence imaging of cryosamples prior to cryoEM data acquisition does not limit the achievable resolution suggests that super-resolution cryo-fluorescence microscopy and in situ structural biology using cryoEM are entirely compatible.
リンク	J Struct Biol / PubMed:37918761
手法	EM (単粒子)
解像度	1.76 - 1.82 Å
構造データ	16783 8cpm EMDB-16783, PDB-8cpm: Human apoferritin after 405 nm laser exposure 手法: EM (単粒子) / 解像度: 1.81 Å 16784 8cps EMDB-16784, PDB-8cps: Human apoferritin 手法: EM (単粒子) / 解像度: 1.82 Å 16785 8cpt EMDB-16785, PDB-8cpt: Human apoferritin after 488 nm laser exposure 手法: EM (単粒子) / 解像度: 1.79 Å 16786 8cpu EMDB-16786, PDB-8cpu: Human apoferritin after 561 nm laser exposure 手法: EM (単粒子) / 解像度: 1.76 Å 16787 8cpv EMDB-16787, PDB-8cpv: Human apoferritin 手法: EM (単粒子) / 解像度: 1.76 Å 16788 8cpw EMDB-16788, PDB-8cpw: Human apoferritin after 405 nm + 488 nm laser exposure in presence of rsEGFP2 手法: EM (単粒子) / 解像度: 1.79 Å 16789 8cpx EMDB-16789, PDB-8cpx: Human apoferritin after 488 nm laser exposure in presence of rsEGFP2 手法: EM (単粒子) / 解像度: 1.76 Å
化合物	ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト)
キーワード	METAL BINDING PROTEIN / Iron binding protein