EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of RNA-induced autoregulation of the DExH-type RNA helicase maleless.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 23, Page 4318-4333.e10, Year 2023
掲載日	2023年12月7日
著者	Pravin Kumar Ankush Jagtap / Marisa Müller / Anna E Kiss / Andreas W Thomae / Karine Lapouge / Martin Beck / Peter B Becker / Janosch Hennig /
PubMed 要旨	RNA unwinding by DExH-type helicases underlies most RNA metabolism and function. It remains unresolved if and how the basic unwinding reaction of helicases is regulated by auxiliary domains. We ...RNA unwinding by DExH-type helicases underlies most RNA metabolism and function. It remains unresolved if and how the basic unwinding reaction of helicases is regulated by auxiliary domains. We explored the interplay between the RecA and auxiliary domains of the RNA helicase maleless (MLE) from Drosophila using structural and functional studies. We discovered that MLE exists in a dsRNA-bound open conformation and that the auxiliary dsRBD2 domain aligns the substrate RNA with the accessible helicase tunnel. In an ATP-dependent manner, dsRBD2 associates with the helicase module, leading to tunnel closure around ssRNA. Furthermore, our structures provide a rationale for blunt-ended dsRNA unwinding and 3'-5' translocation by MLE. Structure-based MLE mutations confirm the functional relevance of our model for RNA unwinding. Our findings contribute to our understanding of the fundamental mechanics of auxiliary domains in DExH helicase MLE, which serves as a model for its human ortholog and potential therapeutic target, DHX9/RHA.
リンク	Mol Cell / PubMed:37989319
手法	EM (単粒子)
解像度	2.86 - 4.24 Å
構造データ	15931 8b9g EMDB-15931, PDB-8b9g: Cryo-EM structure of MLE in complex with ADP:AlF4 and U10 RNA 手法: EM (単粒子) / 解像度: 2.86 Å 15932 8b9i EMDB-15932, PDB-8b9i: Cryo-EM structure of MLE in complex with ADP:AlF4 and UUC RNA 手法: EM (単粒子) / 解像度: 2.95 Å 15933 8b9j EMDB-15933, PDB-8b9j: Cryo-EM structure of MLE in complex with ADP:AlF4 手法: EM (単粒子) / 解像度: 3.45 Å 15934 8b9k EMDB-15934, PDB-8b9k: Cryo-EM structure of MLE in complex with ADP:AlF4 and SL7modUUC RNA 手法: EM (単粒子) / 解像度: 4.04 Å 15935 8b9l EMDB-15935, PDB-8b9l: Cryo-EM structure of MLE 手法: EM (単粒子) / 解像度: 3.45 Å 17703 8pjb EMDB-17703, PDB-8pjb: Cryo-EM structure of MLE in complex with UUC RNA and ADP 手法: EM (単粒子) / 解像度: 3.62 Å 17711 8pjj EMDB-17711, PDB-8pjj: Cryo-EM structure of MLE in complex with SL7UUC RNA and ADP 手法: EM (単粒子) / 解像度: 4.24 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-HOH: WATER / 水 ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	drosophila melanogaster (キイロショウジョウバエ)
キーワード	RNA BINDING PROTEIN (RNA結合タンパク質) / RNA Helicase (ヘリカーゼ) / Drosophila dosage compensation