EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures illustrate step-by-step mitochondrial transcription initiation.
ジャーナル・号・ページ	Nature, Vol. 622, Issue 7984, Page 872-879, Year 2023
掲載日	2023年10月11日
著者	Quinten Goovaerts / Jiayu Shen / Brent De Wijngaert / Urmimala Basu / Smita S Patel / Kalyan Das /
PubMed 要旨	Transcription initiation is a key regulatory step in gene expression during which RNA polymerase (RNAP) initiates RNA synthesis de novo, and the synthesized RNA at a specific length triggers the ...Transcription initiation is a key regulatory step in gene expression during which RNA polymerase (RNAP) initiates RNA synthesis de novo, and the synthesized RNA at a specific length triggers the transition to the elongation phase. Mitochondria recruit a single-subunit RNAP and one or two auxiliary factors to initiate transcription. Previous studies have revealed the molecular architectures of yeast and human mitochondrial RNAP initiation complexes (ICs). Here we provide a comprehensive, stepwise mechanism of transcription initiation by solving high-resolution cryogenic electron microscopy (cryo-EM) structures of yeast mitochondrial RNAP and the transcription factor Mtf1 catalysing two- to eight-nucleotide RNA synthesis at single-nucleotide addition steps. The growing RNA-DNA is accommodated in the polymerase cleft by template scrunching and non-template reorganization, creating stressed intermediates. During early initiation, non-template strand scrunching and unscrunching destabilize the short two- and three-nucleotide RNAs, triggering abortive synthesis. Subsequently, the non-template reorganizes into a base-stacked staircase-like structure supporting processive five- to eight-nucleotide RNA synthesis. The expanded non-template staircase and highly scrunched template in IC8 destabilize the promoter interactions with Mtf1 to facilitate initiation bubble collapse and promoter escape for the transition from initiation to the elongation complex (EC). The series of transcription initiation steps, each guided by the interplay of multiple structural components, reveal a finely tuned mechanism for potential regulatory control.
リンク	Nature / PubMed:37821701 / PubMed Central
手法	EM (単粒子)
解像度	3.39 - 3.75 Å
構造データ	15556 8ap1 EMDB-15556, PDB-8ap1: Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with two GTP molecules poised for de novo initiation (IC2) 手法: EM (単粒子) / 解像度: 3.47 Å 15662 8att EMDB-15662, PDB-8att: Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with 4-mer RNA, pppGpGpUpA (IC4) 手法: EM (単粒子) / 解像度: 3.44 Å 15664 8atv EMDB-15664, PDB-8atv: Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with 5-mer RNA, pppGpGpApApA (IC5) 手法: EM (単粒子) / 解像度: 3.39 Å 15665 8atw EMDB-15665, PDB-8atw: Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with 6-mer RNA, pppGpGpApApApU (IC6) 手法: EM (単粒子) / 解像度: 3.62 Å 16442 8c5s EMDB-16442, PDB-8c5s: Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with 7-mer RNA, pppGpGpUpApApApU (IC7) 手法: EM (単粒子) / 解像度: 3.75 Å 16443 8c5u EMDB-16443, PDB-8c5u: Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with 8-mer RNA, pppGpGpUpApApApUpG (IC8) 手法: EM (単粒子) / 解像度: 3.62 Å 18183 8q63 EMDB-18183, PDB-8q63: Cryo-EM structure of IC8', a second state of yeast mitochondrial RNA polymerase transcription initiation complex with 8-mer RNA, pppGpGpUpApApApUpG 手法: EM (単粒子) / 解像度: 3.68 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM / グアノシン三リン酸
由来	saccharomyces cerevisiae s288c (パン酵母)
キーワード	TRANSCRIPTION (転写 (生物学)) / gene transcription (転写 (生物学)) / polymerase (ポリメラーゼ) / RDRP (RNA依存性RNAポリメラーゼ) / MTF1 / RPO41 / POLRMT / mtRNAP / DNA (デオキシリボ核酸) / transcription initiation (転写 (生物学)) / RNA polymerase (RNAポリメラーゼ) / mitochondria (ミトコンドリア) / IC7 / IC8 / IC8'