EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Systemwide disassembly and assembly of SCF ubiquitin ligase complexes.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 9, Page 1895-1911.e21, Year 2023
掲載日	2023年4月27日
著者	Kheewoong Baek / Daniel C Scott / Lukas T Henneberg / Moeko T King / Matthias Mann / Brenda A Schulman /
PubMed 要旨	Cells respond to environmental cues by remodeling their inventories of multiprotein complexes. Cellular repertoires of SCF (SKP1-CUL1-F box protein) ubiquitin ligase complexes, which mediate much ...Cells respond to environmental cues by remodeling their inventories of multiprotein complexes. Cellular repertoires of SCF (SKP1-CUL1-F box protein) ubiquitin ligase complexes, which mediate much protein degradation, require CAND1 to distribute the limiting CUL1 subunit across the family of ∼70 different F box proteins. Yet, how a single factor coordinately assembles numerous distinct multiprotein complexes remains unknown. We obtained cryo-EM structures of CAND1-bound SCF complexes in multiple states and correlated mutational effects on structures, biochemistry, and cellular assays. The data suggest that CAND1 clasps idling catalytic domains of an inactive SCF, rolls around, and allosterically rocks and destabilizes the SCF. New SCF production proceeds in reverse, through SKP1-F box allosterically destabilizing CAND1. The CAND1-SCF conformational ensemble recycles CUL1 from inactive complexes, fueling mixing and matching of SCF parts for E3 activation in response to substrate availability. Our data reveal biogenesis of a predominant family of E3 ligases, and the molecular basis for systemwide multiprotein complex assembly.
リンク	Cell / PubMed:37028429 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 9.4 Å
構造データ	14561 7z8r EMDB-14561, PDB-7z8r: CAND1-CUL1-RBX1 手法: EM (単粒子) / 解像度: 2.7 Å 14563 7z8t EMDB-14563, PDB-7z8t: CAND1-SCF-SKP2 CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 3.0 Å 14564 7z8v EMDB-14564, PDB-7z8v: CAND1-SCF-SKP2 (SKP1deldel) CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 2.7 Å 14594 7zbw EMDB-14594, PDB-7zbw: CAND1-SCF-SKP2 CAND1 rolling-2 SCF engaged 手法: EM (単粒子) / 解像度: 3.5 Å 14597 7zbz EMDB-14597, PDB-7zbz: CAND1 delhairpin-SCF-SKP2 CAND1 partly engaged SCF partly rocked 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-14598: CAND1 delhairpin-SCF-SKP2 CAND1 rolling SCF engaged 手法: EM (単粒子) / 解像度: 6.8 Å EMDB-14599: CAND1-SCF-FBXW7 CAND1 rolling-1a 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-14600: CAND1-SCF-FBXW7 CAND1 rolling-1b 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-14601: CAND1-SCF-FBXW7 CAND1 rolling-2c 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-14602: CAND1-SCF-FBXW7 CAND1 rolling-2d 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-14603: CAND1-SCF-FBXW7 CAND1 rolling-2a 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-14604: CAND1-SCF-FBXW7 CAND1 rolling-2b 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-14605: CAND1-SCF-FBXW7 CAND1 little CAND1 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-14606: CAND1-SCF-FBXW7 CAND1 no CAND1-1 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-14607: CAND1-SCF-FBXW7 CAND1 no CAND1-2 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-14608: CAND1-SCF-FBXW7 (SKP1deldel) CAND1 engaged no SKP1-Fbp 手法: EM (単粒子) / 解像度: 5.8 Å EMDB-14609: CAND1-SCF-FBXW7 (SKP1deldel) CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-14610: CAND1delhairpin-SCF-FBXW7 CAND1 partly engaged SCF partly rocked 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-14611: CAND1delhairpin-SCF-FBXW7 CAND1 rolling-2d SCF engaged 手法: EM (単粒子) / 解像度: 8.6 Å EMDB-14612: CAND1delhairpin-SCF-FBXW7 CAND1 rolling-2c SCF engaged 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-14613: CAND1delhairpin-SCF-FBXW7 CAND1 rolling-2b SCF engaged 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-14614: CAND1delhairpin-SCF-FBXW7 CAND1 rolling-2a SCF engaged 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-14615: CAND1(2X)-SCF-FBXW7 no CAND1 SCF engaged 手法: EM (単粒子) / 解像度: 8.15 Å EMDB-14616: CAND1(2X)-SCF-FBXW7 CAND1 rolling-1 SCF engaged 手法: EM (単粒子) / 解像度: 7.54 Å 16575 8cdj EMDB-16575, PDB-8cdj: CAND1 b-hairpin++-SCF-SKP2 CAND1 rolling SCF engaged 手法: EM (単粒子) / 解像度: 3.4 Å 16576 8cdk EMDB-16576, PDB-8cdk: CAND1 b-hairpin++-SCF-SKP2 CAND1 partly engaged SCF partly rocked 手法: EM (単粒子) / 解像度: 3.32 Å EMDB-16577: CAND1(2X)-SCF-FBXW7 CAND1 rolling-2 SCF engaged 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-16578: CAND1(2X)-SCF-FBXW7 CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 7.18 Å EMDB-16579: CAND1-SCF-FBXW7 no CAND1 SCF engaged (low res) 手法: EM (単粒子) / 解像度: 8.15 Å EMDB-16580: CAND1-SCF-FBXW7 CAND1 rolling-1 SCF engaged (low res) 手法: EM (単粒子) / 解像度: 8.37 Å EMDB-16581: CAND1-SCF-FBXW7 CAND1 rolling-2 SCF engaged (low res) 手法: EM (単粒子) / 解像度: 6.85 Å EMDB-16582: CAND1-SCF-SKP2 CAND1 rolling-1 SCF engaged 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-16583: CAND1-SCF-SKP2 CAND1 rolling-2 SCF engaged (low res) 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-16584: CAND1-SCF-SKP2 CAND1 engaged SCF rocked (low res) 手法: EM (単粒子) / 解像度: 5.29 Å EMDB-16585: CAND1-SCF-FBXO6 no CAND1 SCF engaged 手法: EM (単粒子) / 解像度: 9.13 Å EMDB-16586: CAND1-SCF-FBXO6 CAND1 rolling-1 SCF engaged 手法: EM (単粒子) / 解像度: 7.35 Å EMDB-16587: CAND1-SCF-FBXO6 CAND1 rolling-2 SCF engaged 手法: EM (単粒子) / 解像度: 7.93 Å EMDB-16588: CAND1-SCF-FBXO6 CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 7.54 Å EMDB-16589: CAND1-SCF-FBXO6 CAND1 engaged no SKP1-Fbp 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-16617: CAND1-SCF-SKP2 with substrate p27-CKSHS1-CyclinA-CDK2, CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 7.73 Å EMDB-16621: CAND1-SCF-SKP2 with substrate p27-CKSHS1-CyclinA-CDK2, CAND1 rolling SCF engaged 手法: EM (単粒子) / 解像度: 8.6 Å EMDB-16622: CAND1-SCF-SKP2 with substrate p27-CKSHS1, CAND1 engaged SCF rocked 手法: EM (単粒子) / 解像度: 7.35 Å EMDB-16623: CAND1-SCF-SKP2 with substrate p27-CKSHS1, CAND1 rolling SCF engaged 手法: EM (単粒子) / 解像度: 9.4 Å EMDB-16625: CAND1-SCF-SKP2 with substrate p27-CKSHS1-CyclinA-CDK2, no CAND1 SCF engaged 手法: EM (単粒子) / 解像度: 7.73 Å EMDB-16764: CAND1delb-hairpin CUL1-RBX1 CAND1 partly engaged 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-16765: CAND1delb-hairpin CUL1-RBX1 CAND1 rolling-1 手法: EM (単粒子) / 解像度: 9.1 Å EMDB-16766: CAND1delb-hairpin CUL1-RBX1 CAND1 rolling-2 手法: EM (単粒子) / 解像度: 7.73 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	LIGASE (リガーゼ) / cullin-RING E3 ubiquitin ligase / Assembly factor / CAND1 / SCF