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-Structure paper
タイトル | SAP domain forms a flexible part of DNA aperture in Ku70/80. |
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ジャーナル・号・ページ | FEBS J, Vol. 288, Issue 14, Page 4382-4393, Year 2021 |
掲載日 | 2021年2月16日 |
著者 | Aleš Hnízda / Petr Tesina / Thanh-Binh Nguyen / Zdeněk Kukačka / Lukas Kater / Amanda K Chaplin / Roland Beckmann / David B Ascher / Petr Novák / Tom L Blundell / |
PubMed 要旨 | Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA ...Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C-terminal domain, known as the SAP domain. Using single-particle cryo-electron microscopy, mass spectrometric analysis of intermolecular cross-linking and molecular modelling simulations, we captured variable positions of the SAP domain depending on DNA binding. The first position was localized at the DNA aperture in the Ku70/80 apo form but was not observed in the DNA-bound state. The second position, which was observed in both apo and DNA-bound states, was found below the DNA aperture, close to the helical arm of Ku70. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. DATABASES: EM maps have been deposited in EMDB (EMD-11933). Coordinates have been deposited in Protein Data Bank (PDB 7AXZ). Other data are available from corresponding authors upon a request. |
リンク | FEBS J / PubMed:33511782 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.2 Å |
構造データ | EMDB-11933, PDB-7axz: |
由来 |
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キーワード | DNA BINDING PROTEIN (DNA結合タンパク質) / NHEJ (非相同末端結合) / Ku70/80 / XRCC5 / XRCC6 / DNA damage (DNA修復) |