EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP.
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 1, Page 115-126.e7, Year 2021
掲載日	2021年1月7日
著者	Caillan Crowe-McAuliffe / Hiraku Takada / Victoriia Murina / Christine Polte / Sergo Kasvandik / Tanel Tenson / Zoya Ignatova / Gemma C Atkinson / Daniel N Wilson / Vasili Hauryliuk /
PubMed 要旨	In all branches of life, stalled translation intermediates are recognized and processed by ribosome-associated quality control (RQC) pathways. RQC begins with the splitting of stalled ribosomes, ...In all branches of life, stalled translation intermediates are recognized and processed by ribosome-associated quality control (RQC) pathways. RQC begins with the splitting of stalled ribosomes, leaving an unfinished polypeptide still attached to the large subunit. Ancient and conserved NEMF family RQC proteins target these incomplete proteins for degradation by the addition of C-terminal "tails." How such tailing can occur without the regular suite of translational components is, however, unclear. Using single-particle cryo-electron microscopy (EM) of native complexes, we show that C-terminal tailing in Bacillus subtilis is mediated by NEMF protein RqcH in concert with RqcP, an Hsp15 family protein. Our structures reveal how these factors mediate tRNA movement across the ribosomal 50S subunit to synthesize polypeptides in the absence of mRNA or the small subunit.
リンク	Mol Cell / PubMed:33259810
手法	EM (単粒子)
解像度	2.6 - 4.9 Å
構造データ	11889 7as8 EMDB-11889, PDB-7as8: Bacillus subtilis ribosome quality control complex state B. Ribosomal 50S subunit with P-tRNA, RqcH, and RqcP/YabO 手法: EM (単粒子) / 解像度: 2.9 Å 11890 7as9 EMDB-11890, PDB-7as9: Bacillus subtilis ribosome-associated quality control complex state A. Ribosomal 50S subunit with peptidyl tRNA in the A/P position and RqcH. 手法: EM (単粒子) / 解像度: 3.5 Å 11891 7asa EMDB-11891, PDB-7asa: Bacillus subtilis ribosome-associated quality control complex state B, multibody refinement focussed on RqcH. Ribosomal 50S subunit with P-tRNA, RqcH, and RqcP/YabO 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-11913: Bacillus subtilis ribosome-associated quality control complex, state C, derived from RqcH affinity purification. 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-11914: Ribosome-associated quality control complex from Bacillus subtilis, state D. Large ribosomal subunit in complex with P-tRNA and RqcP/YabO. 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-11915: Bacillus subtilis ribosome-associated quality control complex state *B, derived from RqcH affinity purification. 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-11916: Bacillus subtilis ribosome-associated quality control complex, state E, derived from RqcP/YabO affinity purification. 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-11917: Bacillus subtilis ribosome-associated quality control complex with SRP, derived from RqcP/YabO affinity purification. 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-11918: Bacillus subtilis ribosome-associated quality control complex, state D, derived from RqcP/YabO affinity purification. 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-11919: Bacillus subtilis ribosome-associated quality control complex, state B, derived from RqcP/YabO affinity purification. 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-11920: Bacillus subtilis ribosome-associated quality control complex with RsfS, derived from RqcP/YabO affinity purification. 手法: EM (単粒子) / 解像度**: 3.1 Å
由来	bacillus subtilis (strain 168) (枯草菌) bacillus subtilis subsp. subtilis str. 168 (枯草菌)
キーワード	TRANSLATION (翻訳 (生物学)) / 50S / tRNA (転移RNA) / RQC / RqcH / peptidyl-tRNA (細菌の翻訳) / RqcP / YabO / alanine tailing